Production of stearidonic acid-rich triacylglycerol via a two-step enzymatic esterification

Nam Ho Kim, Heejin Kim, Nakyung Choi, Yangha Kim, Byung Hee Kim, In Hwan Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


The aim of this study was to synthesize stearidonic acid (SDA)-rich triacylglycerol (TAG) via a two-step lipase-catalyzed esterification under vacuum. SDA-rich fatty acid, which was prepared from echium oil via Candida rugosa lipase-catalyzed selective esterification, was used as the substrate. Two different immobilized lipases, Novozym 435 from Candida antarctica and Lipozyme TL IM from Thermomyces lanuginosus, were employed for the synthesis of SDA-rich TAG. In the first step, Novozym 435-catalyzed esterification of the SDA-rich fatty acid with glycerol was carried out for 2 h. In the second step, Lipozyme TL IM-catalyzed esterification of the reaction mixture from the first step was performed for an additional 10 h. The optimal reaction conditions for the second step were a temperature of 65 °C, an enzyme loading of 20%, and a vacuum of 0.7 kPa. Consequently, the maximum TAG conversion of ca. 86.4 wt% was obtained after 12 h via a two-step lipase-catalyzed esterification.

Original languageEnglish
Pages (from-to)332-337
Number of pages6
JournalFood Chemistry
Publication statusPublished - 2019 Jan 1

Bibliographical note

Funding Information:
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through Agri-Bio industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) ( 117037-2 ).

Publisher Copyright:
© 2018 Elsevier Ltd


  • Esterification
  • Lipase
  • Lipozyme TL IM
  • Novozym 435
  • Stearidonic acid
  • Triacylglycerol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science


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