Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery

Chan Kyum Kim; Han Suk Choi; Sang Jun Lee; Ja Hyun Lee; Ju Hun Lee; Hah Young Yoo; Sung Ok Han; Seung Wook Kim

doi:10.1016/j.procbio.2017.11.001

Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery

Chan Kyum Kim
, Han Suk Choi
, Sang Jun Lee
, Ja Hyun Lee
, Ju Hun Lee
, Hah Young Yoo
, Sung Ok Han
, Seung Wook Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

In this study, the xylanase gene from Cellulomonas flavigena KCTC 9104 was cloned into pPICZαB and expressed in Pichia pastoris X-33. An extracellular endo-1,4-β-xylanase was produced by novel engineered P. pastoris (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38 kDa molecular weight, 55 °C optimum temperature and optimum pH of 6. Under the conditions, the K_m and V_max of rXynCf were 3.6 ± 0.08 mg/mL and 4505 ± 52 μmol/min mg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.

Original language	English
Pages (from-to)	130-135
Number of pages	6
Journal	Process Biochemistry
Volume	65
DOIs	https://doi.org/10.1016/j.procbio.2017.11.001
Publication status	Published - 2018 Feb

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

Biorefinery
Cellulomonas flavigena
Enzymatic hydrolysis
Pichia pastoris
Xylanase

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2017.11.001

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@article{afdfa48ec179488fa5114c74fb99bd06,

title = "Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery",

abstract = "In this study, the xylanase gene from Cellulomonas flavigena KCTC 9104 was cloned into pPICZαB and expressed in Pichia pastoris X-33. An extracellular endo-1,4-β-xylanase was produced by novel engineered P. pastoris (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38 kDa molecular weight, 55 °C optimum temperature and optimum pH of 6. Under the conditions, the Km and Vmax of rXynCf were 3.6 ± 0.08 mg/mL and 4505 ± 52 μmol/min mg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8\%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1\%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.",

keywords = "Biorefinery, Cellulomonas flavigena, Enzymatic hydrolysis, Pichia pastoris, Xylanase",

author = "Kim, \{Chan Kyum\} and Choi, \{Han Suk\} and Lee, \{Sang Jun\} and Lee, \{Ja Hyun\} and Lee, \{Ju Hun\} and Yoo, \{Hah Young\} and Han, \{Sung Ok\} and Kim, \{Seung Wook\}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

month = feb,

doi = "10.1016/j.procbio.2017.11.001",

language = "English",

volume = "65",

pages = "130--135",

journal = "Process Biochemistry",

issn = "1359-5113",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery

AU - Kim, Chan Kyum

AU - Choi, Han Suk

AU - Lee, Sang Jun

AU - Lee, Ja Hyun

AU - Lee, Ju Hun

AU - Yoo, Hah Young

AU - Han, Sung Ok

AU - Kim, Seung Wook

PY - 2018/2

Y1 - 2018/2

N2 - In this study, the xylanase gene from Cellulomonas flavigena KCTC 9104 was cloned into pPICZαB and expressed in Pichia pastoris X-33. An extracellular endo-1,4-β-xylanase was produced by novel engineered P. pastoris (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38 kDa molecular weight, 55 °C optimum temperature and optimum pH of 6. Under the conditions, the Km and Vmax of rXynCf were 3.6 ± 0.08 mg/mL and 4505 ± 52 μmol/min mg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.

AB - In this study, the xylanase gene from Cellulomonas flavigena KCTC 9104 was cloned into pPICZαB and expressed in Pichia pastoris X-33. An extracellular endo-1,4-β-xylanase was produced by novel engineered P. pastoris (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38 kDa molecular weight, 55 °C optimum temperature and optimum pH of 6. Under the conditions, the Km and Vmax of rXynCf were 3.6 ± 0.08 mg/mL and 4505 ± 52 μmol/min mg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.

KW - Biorefinery

KW - Cellulomonas flavigena

KW - Enzymatic hydrolysis

KW - Pichia pastoris

KW - Xylanase

UR - https://www.scopus.com/pages/publications/85033469244

U2 - 10.1016/j.procbio.2017.11.001

DO - 10.1016/j.procbio.2017.11.001

M3 - Article

AN - SCOPUS:85033469244

SN - 1359-5113

VL - 65

SP - 130

EP - 135

JO - Process Biochemistry

JF - Process Biochemistry

ER -

Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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