Programmed cell death 6 (PDCD6) protein interacts with death-associated protein kinase 1 (DAPk1): Additive effect on apoptosis via caspase-3 dependent pathway

Jeong Heon Lee; Seung Bae Rho; Taehoon Chun

doi:10.1007/s10529-005-7869-x

Programmed cell death 6 (PDCD6) protein interacts with death-associated protein kinase 1 (DAPk1): Additive effect on apoptosis via caspase-3 dependent pathway

Jeong Heon Lee, Seung Bae Rho, Taehoon Chun

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Programmed cell death 6 (PDCD6) protein is a 22 kDa EF-hand type Ca ²⁺-binding protein involved in apoptosis. To define the regulating mechanism of PDCD6 activity in the apoptotic pathway, we searched a human ovary cDNA library for a novel PDCD6 binding protein using a yeast two-hybrid system. The selected protein was the human death-associated protein kinase 1 (DAPk1), another protein that functions as a positive mediator of apoptosis. Co-transfection of PDCD6 and DAPk1 cDNA into a tumor cell line accelerated apoptosis via caspase-3 dependent pathway.

Original language	English
Pages (from-to)	1011-1015
Number of pages	5
Journal	Biotechnology letters
Volume	27
Issue number	14
DOIs	https://doi.org/10.1007/s10529-005-7869-x
Publication status	Published - 2005 Jul
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by Korean government (MOEHRT), R08-2003-000-10118-0.

Keywords

Apoptosis
Caspase
Death-associated protein kinase 1
Programmed cell death 6
Yeast two hybrid

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1007/s10529-005-7869-x

Cite this

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title = "Programmed cell death 6 (PDCD6) protein interacts with death-associated protein kinase 1 (DAPk1): Additive effect on apoptosis via caspase-3 dependent pathway",

abstract = "Programmed cell death 6 (PDCD6) protein is a 22 kDa EF-hand type Ca 2+-binding protein involved in apoptosis. To define the regulating mechanism of PDCD6 activity in the apoptotic pathway, we searched a human ovary cDNA library for a novel PDCD6 binding protein using a yeast two-hybrid system. The selected protein was the human death-associated protein kinase 1 (DAPk1), another protein that functions as a positive mediator of apoptosis. Co-transfection of PDCD6 and DAPk1 cDNA into a tumor cell line accelerated apoptosis via caspase-3 dependent pathway.",

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author = "Lee, {Jeong Heon} and Rho, {Seung Bae} and Taehoon Chun",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by Korean government (MOEHRT), R08-2003-000-10118-0.",

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doi = "10.1007/s10529-005-7869-x",

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T2 - Additive effect on apoptosis via caspase-3 dependent pathway

AU - Lee, Jeong Heon

AU - Rho, Seung Bae

AU - Chun, Taehoon

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by Korean government (MOEHRT), R08-2003-000-10118-0.

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N2 - Programmed cell death 6 (PDCD6) protein is a 22 kDa EF-hand type Ca 2+-binding protein involved in apoptosis. To define the regulating mechanism of PDCD6 activity in the apoptotic pathway, we searched a human ovary cDNA library for a novel PDCD6 binding protein using a yeast two-hybrid system. The selected protein was the human death-associated protein kinase 1 (DAPk1), another protein that functions as a positive mediator of apoptosis. Co-transfection of PDCD6 and DAPk1 cDNA into a tumor cell line accelerated apoptosis via caspase-3 dependent pathway.

AB - Programmed cell death 6 (PDCD6) protein is a 22 kDa EF-hand type Ca 2+-binding protein involved in apoptosis. To define the regulating mechanism of PDCD6 activity in the apoptotic pathway, we searched a human ovary cDNA library for a novel PDCD6 binding protein using a yeast two-hybrid system. The selected protein was the human death-associated protein kinase 1 (DAPk1), another protein that functions as a positive mediator of apoptosis. Co-transfection of PDCD6 and DAPk1 cDNA into a tumor cell line accelerated apoptosis via caspase-3 dependent pathway.

KW - Apoptosis

KW - Caspase

KW - Death-associated protein kinase 1

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Programmed cell death 6 (PDCD6) protein interacts with death-associated protein kinase 1 (DAPk1): Additive effect on apoptosis via caspase-3 dependent pathway

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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