Programming effects of maternal stress on the circadian system of adult offspring

Seongsik Yun, Eun Jeong Lee, Han Kyoung Choe, Gi Hoon Son, Kyungjin Kim, Sooyoung Chung

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Maternal stress has long-lasting influences on the brain functions of offspring, and several brain regions have been proposed to mediate such programming. Although perinatal programming of crosstalk between the circadian and stress systems has been proposed, the functional consequences of prenatal stress on the circadian system and the underlying mechanisms remain largely unknown. Therefore, we investigated whether exposing pregnant mice to chronic restraint stress had prolonged effects on the suprachiasmatic nucleus (SCN), which bears the central pacemaker for mammalian circadian rhythms, of offspring. SCN explants from maternally stressed mice exhibited altered cyclic expression patterns of a luciferase reporter under control of the mouse Per1 promoter (mPer1::LUC), which manifested as a decreased amplitude and impaired stability of the rhythm. Bioluminescence imaging at the single-cell level subsequently revealed that impaired synchrony among individual cells was responsible for the impaired rhythmicity. These intrinsic defects appeared to persist during adulthood. Adult male offspring from stressed mothers showed advanced-phase behavioral rhythms with impaired stability as well as altered clock gene expression in the SCN. In addition to affecting the central rhythm, maternal stress also had prolonged influences on the circadian characteristics of the adrenal gland and liver, as determined by circulating corticosterone levels and hepatic glycogen content, and on canonical clock gene mRNA expression in those tissues. Taken together, our findings suggest that the SCN is a key target of the programming effects of maternal stress. The widespread effects of circadian disruptions caused by a misprogrammed clock may have further impacts on metabolic and mental health in later life.

Original languageEnglish
Pages (from-to)473-484
Number of pages12
JournalExperimental and Molecular Medicine
Volume52
Issue number3
DOIs
Publication statusPublished - 2020 Mar 1

Bibliographical note

Funding Information:
This work was supported by the Ministry of Science and ICT through the National Research Foundation of Korea (NRF-2017R1A2A1A05001351 to K.K.; NRF-2014R1A6A3A04054863 to S.C.; and NRF-2016M3C7A1904340 to G.H.S). The mPer1::LUC transgenic mice were a kind gift from Dr. Hitoshi Okamura (Kyoto University, Kyoto, Japan).

Publisher Copyright:
© 2020, The Author(s).

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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