Proliferation-dependent vulnerability of glucose-deprived astrocytes to nitric oxide-induced cytotoxicity

Jung Jin Choi; Won Ki Kim

doi:10.1016/S0304-3940(98)00763-0

Proliferation-dependent vulnerability of glucose-deprived astrocytes to nitric oxide-induced cytotoxicity

Jung Jin Choi, Won Ki Kim

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The present study investigated the vulnerability of glucose-deprived astrocytes to nitric oxide (NO)-induced cytotoxicity. Primary murine cortical astrocyte cultures of different ages were deprived of glucose in the presence of the NO-releasing reagent 3-morpholinosydnonimine. Glucose-deprived astrocytes displayed an age-dependent vulnerability to NO cytotoxicity. However, this difference in vulnerability with age was found to be dependent on initial plating density. The augmented deaths of astrocyte cultures were consistent with the [³H]thymidine incorporation pattern. Thus, rapidly dividing astrocytes were much more susceptible to NO cytotoxicity under glucose-deprived conditions. The data indicate that the vulnerability of glucose-deprived astrocytes to NO cytotoxicity depends on the proliferative state rather than age in vitro.

Original language	English
Pages (from-to)	109-112
Number of pages	4
Journal	Neuroscience Letters
Volume	256
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(98)00763-0
Publication status	Published - 1998 Nov 6
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Korean Science and Engineering Foundation through the Centre for Cell Signalling Research at Ewha Woman's University.

Keywords

Astrocyte
Cell death
Glucose deprivation
Nitric oxide
Primary culture
Proliferation

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0304-3940(98)00763-0

Cite this

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title = "Proliferation-dependent vulnerability of glucose-deprived astrocytes to nitric oxide-induced cytotoxicity",

abstract = "The present study investigated the vulnerability of glucose-deprived astrocytes to nitric oxide (NO)-induced cytotoxicity. Primary murine cortical astrocyte cultures of different ages were deprived of glucose in the presence of the NO-releasing reagent 3-morpholinosydnonimine. Glucose-deprived astrocytes displayed an age-dependent vulnerability to NO cytotoxicity. However, this difference in vulnerability with age was found to be dependent on initial plating density. The augmented deaths of astrocyte cultures were consistent with the [3H]thymidine incorporation pattern. Thus, rapidly dividing astrocytes were much more susceptible to NO cytotoxicity under glucose-deprived conditions. The data indicate that the vulnerability of glucose-deprived astrocytes to NO cytotoxicity depends on the proliferative state rather than age in vitro.",

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AU - Kim, Won Ki

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PY - 1998/11/6

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N2 - The present study investigated the vulnerability of glucose-deprived astrocytes to nitric oxide (NO)-induced cytotoxicity. Primary murine cortical astrocyte cultures of different ages were deprived of glucose in the presence of the NO-releasing reagent 3-morpholinosydnonimine. Glucose-deprived astrocytes displayed an age-dependent vulnerability to NO cytotoxicity. However, this difference in vulnerability with age was found to be dependent on initial plating density. The augmented deaths of astrocyte cultures were consistent with the [3H]thymidine incorporation pattern. Thus, rapidly dividing astrocytes were much more susceptible to NO cytotoxicity under glucose-deprived conditions. The data indicate that the vulnerability of glucose-deprived astrocytes to NO cytotoxicity depends on the proliferative state rather than age in vitro.

AB - The present study investigated the vulnerability of glucose-deprived astrocytes to nitric oxide (NO)-induced cytotoxicity. Primary murine cortical astrocyte cultures of different ages were deprived of glucose in the presence of the NO-releasing reagent 3-morpholinosydnonimine. Glucose-deprived astrocytes displayed an age-dependent vulnerability to NO cytotoxicity. However, this difference in vulnerability with age was found to be dependent on initial plating density. The augmented deaths of astrocyte cultures were consistent with the [3H]thymidine incorporation pattern. Thus, rapidly dividing astrocytes were much more susceptible to NO cytotoxicity under glucose-deprived conditions. The data indicate that the vulnerability of glucose-deprived astrocytes to NO cytotoxicity depends on the proliferative state rather than age in vitro.

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Proliferation-dependent vulnerability of glucose-deprived astrocytes to nitric oxide-induced cytotoxicity

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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