Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

Chul Sang Lee; Pei Lei Tan; Ju Young Eor; Da Hye Choi; Miri Park; Sung Keum Seo; Seokmin Yoon; Siyoung Yang; Sae Hun Kim

doi:10.1002/jsfa.9518

Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

Chul Sang Lee, Pei Lei Tan, Ju Young Eor, Da Hye Choi, Miri Park, Sung Keum Seo, Seokmin Yoon, Siyoung Yang, Sae Hun Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

BACKGROUND: This study investigated the in vivo prophylactic effect of probiotic chocolate on constipation. Rats were administered chocolate containing 2.5 × 10 ¹⁰ CFU g ⁻¹ of probiotics daily for 4 weeks and treated with loperamide (5 mg kg ⁻¹ ) daily at the fourth week of treatment. RESULTS: Probiotic chocolate treatment significantly (P < 0.05) increased the intestinal motility, colon length, fecal moisture content and number of excreted fecal pellets in constipated rats. Moreover, quantitative real-time polymerase chain reaction data and histological images also revealed that both probiotic chocolate LYC and BB12 treatments were capable of upregulating the mRNA expression levels of colonic ZO-1, occludin and AQP8, leading to the maintenance of the defensive barrier function in the constipated rats compared with the negative controls. Interestingly, these treatments also modulated gut bacterial populations by increasing the abundance levels of Lactobacillus and Bifidobacterium, as well as reducing the abundance level of Enterobacteriaceae. CONCLUSION: The present study demonstrated that probiotic chocolate LYC and BB12 could potentially be used as alternative agents for prophylactic constipation.

Original language	English
Pages (from-to)	3045-3056
Number of pages	12
Journal	Journal of the Science of Food and Agriculture
Volume	99
Issue number	6
DOIs	https://doi.org/10.1002/jsfa.9518
Publication status	Published - 2019 Apr

Keywords

constipation
functional chocolate
gastrointestinal function
intestinal barrier function
lactic acid bacteria

ASJC Scopus subject areas

Biotechnology
Food Science
Agronomy and Crop Science
Nutrition and Dietetics

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10.1002/jsfa.9518

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title = "Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats",

abstract = " BACKGROUND: This study investigated the in vivo prophylactic effect of probiotic chocolate on constipation. Rats were administered chocolate containing 2.5 × 10 10 CFU g −1 of probiotics daily for 4 weeks and treated with loperamide (5 mg kg −1 ) daily at the fourth week of treatment. RESULTS: Probiotic chocolate treatment significantly (P < 0.05) increased the intestinal motility, colon length, fecal moisture content and number of excreted fecal pellets in constipated rats. Moreover, quantitative real-time polymerase chain reaction data and histological images also revealed that both probiotic chocolate LYC and BB12 treatments were capable of upregulating the mRNA expression levels of colonic ZO-1, occludin and AQP8, leading to the maintenance of the defensive barrier function in the constipated rats compared with the negative controls. Interestingly, these treatments also modulated gut bacterial populations by increasing the abundance levels of Lactobacillus and Bifidobacterium, as well as reducing the abundance level of Enterobacteriaceae. CONCLUSION: The present study demonstrated that probiotic chocolate LYC and BB12 could potentially be used as alternative agents for prophylactic constipation.",

keywords = "constipation, functional chocolate, gastrointestinal function, intestinal barrier function, lactic acid bacteria",

author = "Lee, {Chul Sang} and Tan, {Pei Lei} and Eor, {Ju Young} and Choi, {Da Hye} and Miri Park and Seo, {Sung Keum} and Seokmin Yoon and Siyoung Yang and Kim, {Sae Hun}",

note = "Funding Information: This work was financially supported by Lotte Confectionery Co. Ltd and Korea University. The authors declare that they have no conflicts of interest. Publisher Copyright: {\textcopyright} 2018 Society of Chemical Industry",

year = "2019",

month = apr,

doi = "10.1002/jsfa.9518",

language = "English",

volume = "99",

pages = "3045--3056",

journal = "Journal of the Science of Food and Agriculture",

issn = "0022-5142",

publisher = "John Wiley and Sons Ltd",

number = "6",

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T1 - Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

AU - Lee, Chul Sang

AU - Tan, Pei Lei

AU - Eor, Ju Young

AU - Choi, Da Hye

AU - Park, Miri

AU - Seo, Sung Keum

AU - Yoon, Seokmin

AU - Yang, Siyoung

AU - Kim, Sae Hun

N1 - Funding Information: This work was financially supported by Lotte Confectionery Co. Ltd and Korea University. The authors declare that they have no conflicts of interest. Publisher Copyright: © 2018 Society of Chemical Industry

PY - 2019/4

Y1 - 2019/4

N2 - BACKGROUND: This study investigated the in vivo prophylactic effect of probiotic chocolate on constipation. Rats were administered chocolate containing 2.5 × 10 10 CFU g −1 of probiotics daily for 4 weeks and treated with loperamide (5 mg kg −1 ) daily at the fourth week of treatment. RESULTS: Probiotic chocolate treatment significantly (P < 0.05) increased the intestinal motility, colon length, fecal moisture content and number of excreted fecal pellets in constipated rats. Moreover, quantitative real-time polymerase chain reaction data and histological images also revealed that both probiotic chocolate LYC and BB12 treatments were capable of upregulating the mRNA expression levels of colonic ZO-1, occludin and AQP8, leading to the maintenance of the defensive barrier function in the constipated rats compared with the negative controls. Interestingly, these treatments also modulated gut bacterial populations by increasing the abundance levels of Lactobacillus and Bifidobacterium, as well as reducing the abundance level of Enterobacteriaceae. CONCLUSION: The present study demonstrated that probiotic chocolate LYC and BB12 could potentially be used as alternative agents for prophylactic constipation.

AB - BACKGROUND: This study investigated the in vivo prophylactic effect of probiotic chocolate on constipation. Rats were administered chocolate containing 2.5 × 10 10 CFU g −1 of probiotics daily for 4 weeks and treated with loperamide (5 mg kg −1 ) daily at the fourth week of treatment. RESULTS: Probiotic chocolate treatment significantly (P < 0.05) increased the intestinal motility, colon length, fecal moisture content and number of excreted fecal pellets in constipated rats. Moreover, quantitative real-time polymerase chain reaction data and histological images also revealed that both probiotic chocolate LYC and BB12 treatments were capable of upregulating the mRNA expression levels of colonic ZO-1, occludin and AQP8, leading to the maintenance of the defensive barrier function in the constipated rats compared with the negative controls. Interestingly, these treatments also modulated gut bacterial populations by increasing the abundance levels of Lactobacillus and Bifidobacterium, as well as reducing the abundance level of Enterobacteriaceae. CONCLUSION: The present study demonstrated that probiotic chocolate LYC and BB12 could potentially be used as alternative agents for prophylactic constipation.

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KW - functional chocolate

KW - gastrointestinal function

KW - intestinal barrier function

KW - lactic acid bacteria

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Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

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