Protease-activated receptor 1-induced GABA release in cultured cortical astrocytes pretreated with GABA is mediated by the Bestrophin-1 channel

C. Justin Lee, Bo Eun Yoon

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

γ-amino butyric acid (GABA) is the main transmitter mediating inhibitory synaptic transmission in the brain and is released not only from a subset of neurons but also from astrocytes. It has been well established that GABA is released via Ca2+- dependent exocytosis of GABA-containing vesicles in neurons. However, a novel form of GABA release in astrocytes via the Ca2+-activated anion channel, Bestrophin-1 (Best1), has been recently reported. In here, we reveal a novel anion channel-mediated GABA release mechanism in cultured cortical astrocytes pretreated with GABA. We have observed that cultured cortical astrocytes do not contain much GABA. We demonstrate in these same astrocytes, pretreated with GABA, that activation of the protease-activated receptor 1 (PAR1) produces an increase in intracellular Ca2+ concentration that leads to opening of Best1 channels and the subsequent release of GABA. These results provide strong molecular evidence for a potential astrocyte-neuron interaction via PAR1-induced and Best1-mediated GABA release.

Original languageEnglish
Pages (from-to)244-249
Number of pages6
JournalAnimal Cells and Systems
Volume18
Issue number4
DOIs
Publication statusPublished - 2014 Jul
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported within the framework of the International Cooperation Program managed by the National Research Foundation of Korea (2013K2A2A4003724), the World Class Institute (WCI 2009-003) programs of the National Research Foundation (NRF) funded by the Korean Ministry of Science, Education and Technology (MEST), by the National Agenda Project (NAP) of the Korea Research Council of Fundamental Science and Technology (KRCF: NAP-09-04), and by an Internal Grant (2E24480) from the Korea Institute of Science and Technology (KIST).

Keywords

  • Bestrophin
  • GABA
  • PAR1

ASJC Scopus subject areas

  • Animal Science and Zoology
  • General Biochemistry,Genetics and Molecular Biology

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