Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish

Moo Kyun Park, Gi Jung Im, Jiwon Chang, Sungwon Chae, Jun Yoo, Won gue Han, Gyu Ho Hwang, Jong Yoon Jung, Jungim Choi, Hak Hyun Jung, Ah Young Chung, Hae Chul Park, June Choi

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.

Original languageEnglish
Pages (from-to)1311-1315
Number of pages5
JournalInternational Journal of Pediatric Otorhinolaryngology
Issue number8
Publication statusPublished - 2014 Aug

Bibliographical note

Funding Information:
This research was supported by a Korea University Grant; by the Soo ENT Clinic and Communication Disorders Center, Korea University and by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2012R1A1A1013003).


  • Caffeic acid phenethyl ester
  • Neomycin
  • Ototoxicity
  • Zebrafish

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology


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