Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish

Moo Kyun Park; Gi Jung Im; Jiwon Chang; Sungwon Chae; Jun Yoo; Won gue Han; Gyu Ho Hwang; Jong Yoon Jung; Jungim Choi; Hak Hyun Jung; Ah Young Chung; Hae Chul Park; June Choi

doi:10.1016/j.ijporl.2014.05.018

Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish

Moo Kyun Park, Gi Jung Im, Jiwon Chang, Sungwon Chae, Jun Yoo, Won gue Han, Gyu Ho Hwang, Jong Yoon Jung, Jungim Choi, Hak Hyun Jung, Ah Young Chung, Hae Chul Park, June Choi

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.

Original language	English
Pages (from-to)	1311-1315
Number of pages	5
Journal	International Journal of Pediatric Otorhinolaryngology
Volume	78
Issue number	8
DOIs	https://doi.org/10.1016/j.ijporl.2014.05.018
Publication status	Published - 2014 Aug

Keywords

Caffeic acid phenethyl ester
Neomycin
Ototoxicity
Zebrafish

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Otorhinolaryngology

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10.1016/j.ijporl.2014.05.018

Cite this

Park, M. K., Im, G. J., Chang, J., Chae, S., Yoo, J., Han, W. G., Hwang, G. H., Jung, J. Y., Choi, J., Jung, H. H., Chung, A. Y., Park, H. C., & Choi, J. (2014). Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish. International Journal of Pediatric Otorhinolaryngology, 78(8), 1311-1315. https://doi.org/10.1016/j.ijporl.2014.05.018

Park, MK, Im, GJ, Chang, J, Chae, S, Yoo, J, Han, WG, Hwang, GH, Jung, JY, Choi, J, Jung, HH, Chung, AY, Park, HC & Choi, J 2014, 'Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish', International Journal of Pediatric Otorhinolaryngology, vol. 78, no. 8, pp. 1311-1315. https://doi.org/10.1016/j.ijporl.2014.05.018

@article{165a3bb3fc304c8c92ad0812eb73624e,

title = "Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish",

abstract = "Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.",

keywords = "Caffeic acid phenethyl ester, Neomycin, Ototoxicity, Zebrafish",

author = "Park, {Moo Kyun} and Im, {Gi Jung} and Jiwon Chang and Sungwon Chae and Jun Yoo and Han, {Won gue} and Hwang, {Gyu Ho} and Jung, {Jong Yoon} and Jungim Choi and Jung, {Hak Hyun} and Chung, {Ah Young} and Park, {Hae Chul} and June Choi",

note = "Funding Information: This research was supported by a Korea University Grant; by the Soo ENT Clinic and Communication Disorders Center, Korea University and by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2012R1A1A1013003).",

year = "2014",

month = aug,

doi = "10.1016/j.ijporl.2014.05.018",

language = "English",

volume = "78",

pages = "1311--1315",

journal = "International Journal of Pediatric Otorhinolaryngology",

issn = "0165-5876",

publisher = "Elsevier Ireland Ltd",

number = "8",

}

TY - JOUR

T1 - Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish

AU - Park, Moo Kyun

AU - Im, Gi Jung

AU - Chang, Jiwon

AU - Chae, Sungwon

AU - Yoo, Jun

AU - Han, Won gue

AU - Hwang, Gyu Ho

AU - Jung, Jong Yoon

AU - Choi, Jungim

AU - Jung, Hak Hyun

AU - Chung, Ah Young

AU - Park, Hae Chul

AU - Choi, June

N1 - Funding Information: This research was supported by a Korea University Grant; by the Soo ENT Clinic and Communication Disorders Center, Korea University and by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2012R1A1A1013003).

PY - 2014/8

Y1 - 2014/8

N2 - Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.

AB - Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.

KW - Caffeic acid phenethyl ester

KW - Neomycin

KW - Ototoxicity

KW - Zebrafish

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U2 - 10.1016/j.ijporl.2014.05.018

DO - 10.1016/j.ijporl.2014.05.018

M3 - Article

C2 - 24880922

AN - SCOPUS:84904049478

SN - 0165-5876

VL - 78

SP - 1311

EP - 1315

JO - International Journal of Pediatric Otorhinolaryngology

JF - International Journal of Pediatric Otorhinolaryngology

IS - 8

ER -

Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish

Abstract

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