Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells

Hye Lim Jung; Sung Yong Yang; Min Cheol Pyo; Chung Oui Hong; Mi Hyun Nam; Jin Won Lee; Kwang Won Lee

doi:10.1007/s10068-018-0477-z

Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells

Hye Lim Jung, Sung Yong Yang, Min Cheol Pyo, Chung Oui Hong, Mi Hyun Nam, Jin Won Lee, Kwang Won Lee

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Although chebulic acid isolated from Terminalia chebular has diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by tert-butyl hydroperoxide (t-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in t-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on t-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.

Original language	English
Pages (from-to)	555-562
Number of pages	8
Journal	Food Science and Biotechnology
Volume	28
Issue number	2
DOIs	https://doi.org/10.1007/s10068-018-0477-z
Publication status	Published - 2019 Apr 5

Keywords

Chebulic acid
Heme oxygenase-1
Mitogen-activated protein kinases
Nuclear factor erythroid 2-related factor 2
γ-Glutamate cysteine ligase

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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10.1007/s10068-018-0477-z

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title = "Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells",

abstract = "Although chebulic acid isolated from Terminalia chebular has diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by tert-butyl hydroperoxide (t-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in t-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on t-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.",

keywords = "Chebulic acid, Heme oxygenase-1, Mitogen-activated protein kinases, Nuclear factor erythroid 2-related factor 2, γ-Glutamate cysteine ligase",

author = "Jung, {Hye Lim} and Yang, {Sung Yong} and Pyo, {Min Cheol} and Hong, {Chung Oui} and Nam, {Mi Hyun} and Lee, {Jin Won} and Lee, {Kwang Won}",

note = "Funding Information: Acknowledgements This work was supported by a Korea University Grant (K1617111). The authors also thank the Institute of Biomedical Science and Food Safety, CJ-Korea University Food Safety Hall (Seoul, South Korea) for providing the equipment and facilities. Publisher Copyright: {\textcopyright} 2018, The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature.",

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doi = "10.1007/s10068-018-0477-z",

language = "English",

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journal = "Food Science and Biotechnology",

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T1 - Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells

AU - Jung, Hye Lim

AU - Yang, Sung Yong

AU - Pyo, Min Cheol

AU - Hong, Chung Oui

AU - Nam, Mi Hyun

AU - Lee, Jin Won

AU - Lee, Kwang Won

N1 - Funding Information: Acknowledgements This work was supported by a Korea University Grant (K1617111). The authors also thank the Institute of Biomedical Science and Food Safety, CJ-Korea University Food Safety Hall (Seoul, South Korea) for providing the equipment and facilities. Publisher Copyright: © 2018, The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature.

PY - 2019/4/5

Y1 - 2019/4/5

N2 - Although chebulic acid isolated from Terminalia chebular has diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by tert-butyl hydroperoxide (t-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in t-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on t-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.

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Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells

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