Abstract
Although chebulic acid isolated from Terminalia chebular has diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by tert-butyl hydroperoxide (t-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in t-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on t-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.
| Original language | English |
|---|---|
| Pages (from-to) | 555-562 |
| Number of pages | 8 |
| Journal | Food Science and Biotechnology |
| Volume | 28 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2019 Apr 5 |
Bibliographical note
Publisher Copyright:© 2018, The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature.
Keywords
- Chebulic acid
- Heme oxygenase-1
- Mitogen-activated protein kinases
- Nuclear factor erythroid 2-related factor 2
- γ-Glutamate cysteine ligase
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology