Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter

Kyung Won Lee; Mi Hyun Nam; Hee Ra Lee; Chung Oui Hong; Kwang Won Lee

doi:10.1186/s12906-017-1870-5

Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter

Kyung Won Lee, Mi Hyun Nam, Hee Ra Lee, Chung Oui Hong, Kwang Won Lee

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.

Original language	English
Article number	373
Journal	BMC Complementary and Alternative Medicine
Volume	17
Issue number	1
DOIs	https://doi.org/10.1186/s12906-017-1870-5
Publication status	Published - 2017 Jul 19

Bibliographical note

Funding Information:
This research was supported by a Korea University Grant (K1420071). This study was also supported by School of Life Sciences and Biotechnology for BK21 PLUS, Korea University. The authors thank the Institute of Biomedical Science & Food Safety, Korea University Food Safety Hall, for providing the equipment and facilities. The funding source had no role in the design, conduct, or analysis of the study or in the decision to submit the manuscript for publication.

Publisher Copyright:
© 2017 The Author(s).

Keywords

Alveolar barrier dysfunction
Chebulic acid
Inflammation
Pulmonary alveolus
Urban particulate matter

ASJC Scopus subject areas

Complementary and alternative medicine

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10.1186/s12906-017-1870-5

Cite this

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title = "Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter",

abstract = "Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.",

keywords = "Alveolar barrier dysfunction, Chebulic acid, Inflammation, Pulmonary alveolus, Urban particulate matter",

author = "Lee, {Kyung Won} and Nam, {Mi Hyun} and Lee, {Hee Ra} and Hong, {Chung Oui} and Lee, {Kwang Won}",

note = "Funding Information: This research was supported by a Korea University Grant (K1420071). This study was also supported by School of Life Sciences and Biotechnology for BK21 PLUS, Korea University. The authors thank the Institute of Biomedical Science & Food Safety, Korea University Food Safety Hall, for providing the equipment and facilities. The funding source had no role in the design, conduct, or analysis of the study or in the decision to submit the manuscript for publication. Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

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doi = "10.1186/s12906-017-1870-5",

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T1 - Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter

AU - Lee, Kyung Won

AU - Nam, Mi Hyun

AU - Lee, Hee Ra

AU - Hong, Chung Oui

AU - Lee, Kwang Won

N1 - Funding Information: This research was supported by a Korea University Grant (K1420071). This study was also supported by School of Life Sciences and Biotechnology for BK21 PLUS, Korea University. The authors thank the Institute of Biomedical Science & Food Safety, Korea University Food Safety Hall, for providing the equipment and facilities. The funding source had no role in the design, conduct, or analysis of the study or in the decision to submit the manuscript for publication. Publisher Copyright: © 2017 The Author(s).

PY - 2017/7/19

Y1 - 2017/7/19

N2 - Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.

AB - Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.

KW - Alveolar barrier dysfunction

KW - Chebulic acid

KW - Inflammation

KW - Pulmonary alveolus

KW - Urban particulate matter

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DO - 10.1186/s12906-017-1870-5

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SN - 1472-6882

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JO - BMC Complementary and Alternative Medicine

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M1 - 373

ER -

Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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