Protective role of NecroX-5 against neomycin-induced hair cell damage in zebrafish

June Choi, Jae-Jun Song, Jiwon Chang, Jungim Choi, Gi Jung Im, Sungwon Chae, Seung Hoon Lee, Soon Young Kwon, Hak Hyun Jung, Ah Young Chung, Hae Chul Park

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


NecroX-5, one of the derivatives of NecroX series compounds, is a mitochondrial reactive oxygen species and reactive nitrogen species scavenger that inhibits cell death against various kinds of oxidative stresses. The objective of the present study was to evaluate the effects of NecroX-5 on neomycin-induced ototoxicity in transgenic zebrafish (Brn3C: EGFP). Five days post-fertilization, zebrafish larvae were exposed to 125 μM neomycin and one of the following NecroX-5 concentrations for 1 h: 10, 25, 50, and 75 μM. Hair cells within the neuromasts of the supraorbital (SO1 and SO2), otic (O1), and occipital (OC1) lateral lines were analyzed using fluorescence microscopy (n = 10). The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay and 2-[4-(dimethylamino) styryl]-N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Ultrastructural changes were evaluated using scanning electron microscopy. NecroX-5 decreased neomycin-induced hair cell loss in the neuromasts (NecroX-5 50 μM: 13.4 ± 2.0 cells, 125 μM neomycin only: 8.1 ± 1.2 cells; n = 10, P < 0.05) and decreased the TUNEL reaction. The ultrastructural analysis showed that the structures of mitochondria and hair cells within the neuromasts were preserved in zebrafish exposed to 125 μM neomycin and 50 μM NecroX-5. NecroX-5 decreased apoptosis and mitochondrial damage. In conclusion, NecroX-5 attenuated neomycin-induced hair cell loss in zebrafish.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalArchives of Toxicology
Issue number2
Publication statusPublished - 2014 Feb


  • NecroX-5
  • Neomycin
  • Ototoxicity
  • Zebrafish

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis


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