Protein Digestibility of Alkali- and Fructose-Treated Protein by Rat True Digestibility Assay and by the Immobilized Digestive Enzyme Assay System

Hyo I. Chang; George L. Catignani; Harold E. Swaisgood

doi:10.1021/jf00094a022

Protein Digestibility of Alkali- and Fructose-Treated Protein by Rat True Digestibility Assay and by the Immobilized Digestive Enzyme Assay System

Hyo I. Chang, George L. Catignani, Harold E. Swaisgood

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The effect of processing on the digestibility of various food proteins was examined by using the immobilized digestive enzyme assay (IDEA) system. The values obtained were compared to true digestibilities determined by rat bioassay. Sodium caseinate, egg white, soy protein, and whey were treated with either 0.2 N NaOH at 40 °C for 6 h or 0.5 M fructose (pH 7.0) at 90 °C for 4 h. Untreated proteins were also analyzed. Treatment of all samples (n = 12) with 4 M urea assured solubility. Regression analysis of data for all samples resulted in a correlation coefficient (r) of 0.83 (p < 0.001). The IDEA systems appears to be an accurate and reliable estimate of in vivo digestibilities. Furthermore, it offers a more rapid and less expensive alternative to animal bioassays.

Original language	English
Pages (from-to)	1016-1018
Number of pages	3
Journal	Journal of agricultural and food chemistry
Volume	38
Issue number	4
DOIs	https://doi.org/10.1021/jf00094a022
Publication status	Published - 1990 Apr

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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title = "Protein Digestibility of Alkali- and Fructose-Treated Protein by Rat True Digestibility Assay and by the Immobilized Digestive Enzyme Assay System",

abstract = "The effect of processing on the digestibility of various food proteins was examined by using the immobilized digestive enzyme assay (IDEA) system. The values obtained were compared to true digestibilities determined by rat bioassay. Sodium caseinate, egg white, soy protein, and whey were treated with either 0.2 N NaOH at 40 °C for 6 h or 0.5 M fructose (pH 7.0) at 90 °C for 4 h. Untreated proteins were also analyzed. Treatment of all samples (n = 12) with 4 M urea assured solubility. Regression analysis of data for all samples resulted in a correlation coefficient (r) of 0.83 (p < 0.001). The IDEA systems appears to be an accurate and reliable estimate of in vivo digestibilities. Furthermore, it offers a more rapid and less expensive alternative to animal bioassays.",

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year = "1990",

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T1 - Protein Digestibility of Alkali- and Fructose-Treated Protein by Rat True Digestibility Assay and by the Immobilized Digestive Enzyme Assay System

AU - Chang, Hyo I.

AU - Catignani, George L.

AU - Swaisgood, Harold E.

PY - 1990/4

Y1 - 1990/4

N2 - The effect of processing on the digestibility of various food proteins was examined by using the immobilized digestive enzyme assay (IDEA) system. The values obtained were compared to true digestibilities determined by rat bioassay. Sodium caseinate, egg white, soy protein, and whey were treated with either 0.2 N NaOH at 40 °C for 6 h or 0.5 M fructose (pH 7.0) at 90 °C for 4 h. Untreated proteins were also analyzed. Treatment of all samples (n = 12) with 4 M urea assured solubility. Regression analysis of data for all samples resulted in a correlation coefficient (r) of 0.83 (p < 0.001). The IDEA systems appears to be an accurate and reliable estimate of in vivo digestibilities. Furthermore, it offers a more rapid and less expensive alternative to animal bioassays.

AB - The effect of processing on the digestibility of various food proteins was examined by using the immobilized digestive enzyme assay (IDEA) system. The values obtained were compared to true digestibilities determined by rat bioassay. Sodium caseinate, egg white, soy protein, and whey were treated with either 0.2 N NaOH at 40 °C for 6 h or 0.5 M fructose (pH 7.0) at 90 °C for 4 h. Untreated proteins were also analyzed. Treatment of all samples (n = 12) with 4 M urea assured solubility. Regression analysis of data for all samples resulted in a correlation coefficient (r) of 0.83 (p < 0.001). The IDEA systems appears to be an accurate and reliable estimate of in vivo digestibilities. Furthermore, it offers a more rapid and less expensive alternative to animal bioassays.

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Protein Digestibility of Alkali- and Fructose-Treated Protein by Rat True Digestibility Assay and by the Immobilized Digestive Enzyme Assay System

Abstract

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