Abstract
Silane coupling agents have been widely used for immobilizing proteins onto inorganic surfaces. However, the immobilization method using silane coupling agents requires several treatment steps, and its application is limited to only surfaces containing hydroxyl groups. The aim of this study was to develop a novel method to overcome the limitations of the silane-based immobilization method using a polymer-bound isocyanate. Initially, polymer-bound isocyanate was dissolved in organic solvent and then was used to dip-coat inorganic surfaces. Proteins were then immobilized onto the dip-coated surfaces by the formation of urea bonds between the isocyanate groups of the polymer and the amine groups of the protein. The reaction was verified by FT-IR in which NCO stretching peaks disappeared, and CO and NH stretching peaks appeared after immobilization. The immobilization efficiency of the newly developed method was insensitive to reaction temperatures (4-50 °C), but the efficiency increased with reaction time and reached a maximum after 4 h. Furthermore, the method showed comparable immobilization efficiency to the silane-based immobilization method and was applicable to surfaces that cannot form hydroxyl groups. Taken together, the newly developed method provides a simple and efficient platform for immobilizing proteins onto surfaces.
Original language | English |
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Pages (from-to) | 198-204 |
Number of pages | 7 |
Journal | Applied Surface Science |
Volume | 324 |
DOIs | |
Publication status | Published - 2015 Jan 1 |
Bibliographical note
Funding Information:This study was supported by the Korea Ministry of Environment as “Projects for Developing Eco-Innovation Technologies ( GT-11-G-02-001-1 )”. We would like to thank Hyomin Ahn at Sehwa High School and Samantha Reuter for proof reading this manuscript, and the anonymous reviewers for their careful comments on the manuscript.
Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
Keywords
- Coating
- Polymer bound isocyanate
- Protein immobilization
- Silane coupling agent
- Surface immobilization
ASJC Scopus subject areas
- General Chemistry
- Condensed Matter Physics
- General Physics and Astronomy
- Surfaces and Interfaces
- Surfaces, Coatings and Films