Protein-inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in l-xylulose production

Sanjay K.S. Patel, Sachin V. Otari, Yun Chan Kang, Jung Kul Lee

    Research output: Contribution to journalArticlepeer-review

    94 Citations (Scopus)

    Abstract

    The facile synthesis of protein-inorganic hybrid nanoflowers was evaluated for the efficient immobilization of recombinant his-tagged enzymes, which have a broad range of potential applications. In this study, we report the preparation of a metal-protein hybrid nanoflower system for efficient immobilization of the recombinant enzymes l-arabinitol 4-dehydrogenase from Hypocrea jecorina (HjLAD) and NADH oxidase from Streptococcus pyogenes (SpNox). Compared with free enzymes, synthesized hybrid nanoflowers exhibited enhanced enzymatic activities of 246 and 144% for HjLAD and SpNox, respectively. We have demonstrated that immobilized enzymes retained high catalytic activity and improved the tolerance towards pH and temperature changes. Synthesized nanoflowers also retained high storage stability and reusability. In addition, the immobilized enzymes exhibited significantly enhanced l-xylulose production under co-factor regeneration conditions than the free enzyme combination. These results demonstrate that variations in the concentration of metals and synthesis conditions of nanoflowers can be extended to efficiently immobilize recombinant his-tagged enzymes.

    Original languageEnglish
    Pages (from-to)3488-3494
    Number of pages7
    JournalRSC Advances
    Volume7
    Issue number6
    DOIs
    Publication statusPublished - 2017

    Bibliographical note

    Funding Information:
    This work was supported by the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (20153030091450). This research was also supported by a grant from the Intelligent Synthetic Biology Center of Global Frontier Project (2013M3A6A8073184) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea. This research was supported by the 2015 KU Brain Pool of Konkuk University.

    Publisher Copyright:
    © The Royal Society of Chemistry.

    ASJC Scopus subject areas

    • General Chemistry
    • General Chemical Engineering

    Fingerprint

    Dive into the research topics of 'Protein-inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in l-xylulose production'. Together they form a unique fingerprint.

    Cite this