Proteomic analysis of glomeruli from streptozotocin-induced diabetic rats

Hyun Jung Kim, O. Deuk Kwon, Sang Hoon Kim, Patrick Taejoon Hwang, Chan Wha Kim

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    The kidney glomeruli are the sites of plasma filtration and production of primary urine. However, they are also the locus of kidney diseases, which progress to chronic renal failure. Glomeruli are a major target of injury in diabetic nephropathy (DN). The mechanisms by which glomerular filtration are regulated are poorly understood, and proteomic investigations of isolated glomeruli on the progressive development of DN in animal models have not been determined. To understand the molecular mechanism leading to DN, especially the glomerular injury mechanism, the differences in the glomerular proteomes of streptozotocin (STZ)-induced- and non-diabetic rats at six and 24 weeks were analyzed via two-dimensional electrophoresis (2-DE). To identify the progressive stages of DN, body weight, blood glucose, and proteinuria were measured periodically, and pathological changes were evaluated by periodic acid-Schiff staining. Magnetic beads were used to isolate glomeruli from kidneys and the glomerular proteomes of non-diabetic and STZ-induced diabetic rats were analyzed by 2-DE and nano-LC-ESI-MS/MS. Glutathione peroxidase 3, peroxiredoxin 2, and histone H2A were down-regulated, and annexin A3 was up-regulated, in the STZ-induced group compared with the controls. Glutathione peroxidase 3 and annexin A3, which might help elucidate the mechanism of DN, were verified by Western blotting. These proteins could potentially provide insight into the mechanism of glomerular injury in DN.

    Original languageEnglish
    Pages (from-to)650-659
    Number of pages10
    JournalBiotechnology and Bioprocess Engineering
    Volume19
    Issue number4
    DOIs
    Publication statusPublished - 2014 Jul 1

    Bibliographical note

    Funding Information:
    This work was supported by a Korea University Grant.

    Publisher Copyright:
    © 2014 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.

    Keywords

    • annexin A3
    • diabetic nephropathy
    • glomerulus
    • glutathione peroxidase 3
    • two-dimensional electrophoresis

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Biomedical Engineering

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