Proteomic analysis of pulmonary sclerosing hemangioma

Lian Jin Jin, Bong Kyung Shin, Woon Yong Jung, Hyun Juu Lee, Su Jin Cho, Joung Ho Han, Seong Yeon Ha, Ae Ree Kim, Young Sik Kim, In Sun Kim, Chang Sub Uhm, Han Kyeom Kim

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been dear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.

    Original languageEnglish
    Pages (from-to)4877-4883
    Number of pages7
    JournalProteomics
    Volume6
    Issue number17
    DOIs
    Publication statusPublished - 2006 Sept

    Keywords

    • 2-D PAGE
    • GST
    • Prx II
    • Sclerosing hemangioma

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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