TY - JOUR
T1 - Pseudomonas neustonica sp. Nov., isolated from the sea surface microlayer of the ross sea (antarctica)
AU - Jang, Gwang Il
AU - Lee, Inae
AU - Ha, Tran Thu
AU - Yoon, Soo Jung
AU - Hwang, Yeon Ju
AU - Yi, Hana
AU - Yun, Sukyoung
AU - Lee, Won Sang
AU - Hwang, Chung Yeon
N1 - Funding Information:
This work was supported mainly by a research grant from the Korean Ministry of Oceans and Fisheries (KIMST20190361; PM19020), Korea and partly by a research grant from National Institute of Fisheries Science (R2020060).
Publisher Copyright:
© 2020, Microbiology Society. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Gram-stain-negative, aerobic and rod-shaped bacterial strains, designated SSM26T and SSM44, were isolated from a sea surface microlayer sample from the Ross Sea, Antarctica. Analysis of the 16S rRNA gene sequences of strains SSM26T and SSM44 revealed a clear affiliation with the genus Pseudomonas. Based on the results of phylogenetic analysis, strains SSM26T and SSM44 showed the closest phylogenetic relationship with the species Pseudomonas sabulinigri KCTC 22137T with the 16S rRNA gene sequence similarity level of 98.5 %. Strains SSM26T and SSM44 grew optimally at 30 °C, pH 7.0–7.5 and 0.5– 10.0 % NaCl (w/v). The major cellular fatty acids were C18: 1 ω7c (31.3–34.9%), C16: 0 (15.5–20.2%), summed feature 3 (C16: 1 ω7c/ C16: 1 ω6c; 19.5–25.4 %) and C12: 0 (6.0–9.3 %). The genomic DNA G+C content of each strain was 56.2 mol%. Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strains SSM26T and SSM44 constituted a single species that was clearly distinguishable from its phylogenetically close relatives. The combined phenotypic, chemotaxonomic, genomic and phylogenetic data also showed that strains SSM26T and SSM44 could be distinguished from validly published members of the genus Pseudomonas. Thus, these strains should be classified as representing a novel species in the genus Pseudomonas, for which the name Pseudomonas neustonica sp. nov. is proposed with the type strain SSM26T (=KCCM 43193T=JCM 31284T=PAMC 28426T) and a sister strain SSM44 (=KCCM 43194=JCM 31285=PAMC 28427).
AB - Gram-stain-negative, aerobic and rod-shaped bacterial strains, designated SSM26T and SSM44, were isolated from a sea surface microlayer sample from the Ross Sea, Antarctica. Analysis of the 16S rRNA gene sequences of strains SSM26T and SSM44 revealed a clear affiliation with the genus Pseudomonas. Based on the results of phylogenetic analysis, strains SSM26T and SSM44 showed the closest phylogenetic relationship with the species Pseudomonas sabulinigri KCTC 22137T with the 16S rRNA gene sequence similarity level of 98.5 %. Strains SSM26T and SSM44 grew optimally at 30 °C, pH 7.0–7.5 and 0.5– 10.0 % NaCl (w/v). The major cellular fatty acids were C18: 1 ω7c (31.3–34.9%), C16: 0 (15.5–20.2%), summed feature 3 (C16: 1 ω7c/ C16: 1 ω6c; 19.5–25.4 %) and C12: 0 (6.0–9.3 %). The genomic DNA G+C content of each strain was 56.2 mol%. Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strains SSM26T and SSM44 constituted a single species that was clearly distinguishable from its phylogenetically close relatives. The combined phenotypic, chemotaxonomic, genomic and phylogenetic data also showed that strains SSM26T and SSM44 could be distinguished from validly published members of the genus Pseudomonas. Thus, these strains should be classified as representing a novel species in the genus Pseudomonas, for which the name Pseudomonas neustonica sp. nov. is proposed with the type strain SSM26T (=KCCM 43193T=JCM 31284T=PAMC 28426T) and a sister strain SSM44 (=KCCM 43194=JCM 31285=PAMC 28427).
KW - Antarctica
KW - Marine bacteria
KW - Pseudomonas
KW - Sea surface microlayer
UR - http://www.scopus.com/inward/record.url?scp=85087378577&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004240
DO - 10.1099/ijsem.0.004240
M3 - Article
C2 - 32511084
AN - SCOPUS:85087378577
SN - 1466-5026
VL - 70
SP - 3832
EP - 3838
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 6
M1 - 004240
ER -