PTRF/cavin-1 is essential for multidrug resistance in cancer cells

Jae Sung Yi; Dong Gi Mun; Hyun Lee; Jun Sub Park; Jung Woo Lee; Jae Seon Lee; Su Jin Kim; Bong Rae Cho; Sang Won Lee; Young Gyu Ko

doi:10.1021/pr300651m

PTRF/cavin-1 is essential for multidrug resistance in cancer cells

Jae Sung Yi, Dong Gi Mun, Hyun Lee, Jun Sub Park, Jung Woo Lee, Jae Seon Lee, Su Jin Kim, Bong Rae Cho, Sang Won Lee, Young Gyu Ko

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand the underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein, caveolin-1, and serum deprivation protein response/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.

Original language	English
Pages (from-to)	605-614
Number of pages	10
Journal	Journal of Proteome Research
Volume	12
Issue number	2
DOIs	https://doi.org/10.1021/pr300651m
Publication status	Published - 2013 Feb 1

Keywords

PTRF
cavin-1
label-free quantitative proteomics
lipid rafts
multidrug resistance

ASJC Scopus subject areas

General Chemistry
Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/pr300651m

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title = "PTRF/cavin-1 is essential for multidrug resistance in cancer cells",

abstract = "Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand the underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein, caveolin-1, and serum deprivation protein response/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.",

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author = "Yi, {Jae Sung} and Mun, {Dong Gi} and Hyun Lee and Park, {Jun Sub} and Lee, {Jung Woo} and Lee, {Jae Seon} and Kim, {Su Jin} and Cho, {Bong Rae} and Lee, {Sang Won} and Ko, {Young Gyu}",

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AU - Yi, Jae Sung

AU - Mun, Dong Gi

AU - Lee, Hyun

AU - Park, Jun Sub

AU - Lee, Jung Woo

AU - Lee, Jae Seon

AU - Kim, Su Jin

AU - Cho, Bong Rae

AU - Lee, Sang Won

AU - Ko, Young Gyu

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand the underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein, caveolin-1, and serum deprivation protein response/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.

AB - Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand the underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein, caveolin-1, and serum deprivation protein response/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.

KW - PTRF

KW - cavin-1

KW - label-free quantitative proteomics

KW - lipid rafts

KW - multidrug resistance

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PTRF/cavin-1 is essential for multidrug resistance in cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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