Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing

Yue Chen; Aram J. Chung; Ting Hsiang Wu; Michael A. Teitell; Dino Di Carlo; Pei Yu Chiou

doi:10.1002/smll.201302885

Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing

Yue Chen
, Aram J. Chung
, Ting Hsiang Wu
, Michael A. Teitell
, Dino Di Carlo
, Pei Yu Chiou^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s^-1 without severe dilution effects from high volume sheath flows.

Original language	English
Pages (from-to)	1746-1751
Number of pages	6
Journal	Small
Volume	10
Issue number	9
DOIs	https://doi.org/10.1002/smll.201302885
Publication status	Published - 2014 May 14
Externally published	Yes

Keywords

cell sorting
inertial focusing
lab-on-a-chip devices
lasers
microfluidics

ASJC Scopus subject areas

Biotechnology
General Chemistry
Biomaterials
General Materials Science
Engineering (miscellaneous)

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10.1002/smll.201302885

Cite this

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abstract = "A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90\% is achieved at a throughput of 10 000 particles s-1 without severe dilution effects from high volume sheath flows.",

keywords = "cell sorting, inertial focusing, lab-on-a-chip devices, lasers, microfluidics",

author = "Yue Chen and Chung, \{Aram J.\} and Wu, \{Ting Hsiang\} and Teitell, \{Michael A.\} and \{Di Carlo\}, Dino and Chiou, \{Pei Yu\}",

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AU - Wu, Ting Hsiang

AU - Teitell, Michael A.

AU - Di Carlo, Dino

AU - Chiou, Pei Yu

PY - 2014/5/14

Y1 - 2014/5/14

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AB - A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s-1 without severe dilution effects from high volume sheath flows.

KW - cell sorting

KW - inertial focusing

KW - lab-on-a-chip devices

KW - lasers

KW - microfluidics

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DO - 10.1002/smll.201302885

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Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing

Abstract

Keywords

ASJC Scopus subject areas

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