Purification and characterization of a cold-active lipase from Pichia lynferdii Y-7723: pH-dependant activity deviation

Jae Han Bae, Mi Hyun Kwon, In Hwan Kim, Ching T. Hou, Hak Ryul Kim

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    Lipases with abnormal functionalities such as high thermostability and optimal activity at extreme conditions gain special attentions because of their applicability in the restricted reaction conditions. In particular, coldactive lipases have gained special attentions in various industrial fields such as washer detergent, pharmaceutical catalyst, and production of structured lipid. However, production of cold-active lipase is mostly found from psychrophilic microorganisms. Recently we found a novel cold-active lipase from Pichia lynferdii Y-7723 which is mesophilic yeast strain. In this study, we purified the cold active lipase and the enzyme was further characterized in several parameters. The enzyme was purified with 33 purification fold using chromatographic techniques and the purified lipase represented maximum lipolytic activity at 15°C and the maximum activity was highly dependent on pH.

    Original languageEnglish
    Pages (from-to)851-857
    Number of pages7
    JournalBiotechnology and Bioprocess Engineering
    Volume19
    Issue number5
    DOIs
    Publication statusPublished - 2014 Nov 20

    Bibliographical note

    Funding Information:
    This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0023701).

    Publisher Copyright:
    © 2014, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.

    Keywords

    • Pichia lynferdii
    • characterization
    • cold-active
    • lipase
    • pH-dependency

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Biomedical Engineering

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