Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp. KH-95

Min Jung Song, Jungdon Bae, Dae Sil Lee, Chang Ho Kim, Jun Seok Kim, Seung Wook Kim, Suk In Hong

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)


To date, prodigiosin and its analogues which have been shown to have anticancer, cytotoxic and immunosuppressive activities have been isolated from Serratia, Pseudomonas and Streptomyces species, and chemically synthesized. In a previous study, the red pigment content in Serratia sp. KH-95 was enhanced using a casein-enriched medium. Recently, an integrated bioreactor with an internal adsorbent has been developed to increase the production yield and allow easy recovery of the pigment. Thus, this study focused on both purifying and identifying a single red pigment from several pigments attached to the adsorbent in an integrated bioreactor. The red pigment was extracted directly from the internal adsorbent using acidified methanol and phase separation. Subsequently, it was purified by silica gel chromatography and high performance liquid chromatograph (HPLC). As a result, pure prodigiosin was identified by structural studies as a pigment. Also, this downstream procedure that uses the integrated bioreactor can be applied to the direct production and purification of other prodigiosin analogues and hydrophobic alkaloid compounds from several microorganisms.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalJournal of Bioscience and Bioengineering
Issue number2
Publication statusPublished - 2006 Feb

Bibliographical note

Funding Information:
This work was financially supported by an Interdisciplinary Research Grant from KSEF under project no. R01-2002-000-00591-0. It was accomplished during Professor Hong’s sabbatical leave.


  • Serratia
  • integrated bioreactor
  • internal adsorbent
  • pigment
  • prodigiosin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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