TY - JOUR
T1 - Pyrosequencing analysis of subgingival microbiota in distinct periodontal conditions
AU - Park, O. J.
AU - Yi, H.
AU - Jeon, J. H.
AU - Kang, S. S.
AU - Koo, K. T.
AU - Kum, K. Y.
AU - Chun, J.
AU - Yun, C. H.
AU - Han, S. H.
N1 - Funding Information:
This work was supported by grants from the National Research Foundation of Korea funded by the Korean government (MISIP) ( 2010-0029116 and 2010-0024083 ); the Agriculture, Food and Rural Affairs Research Center Support Program; the Ministry of Agriculture, Food and Rural Affairs; and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare ( HI14C0469 ), Republic of Korea.
Publisher Copyright:
© International & American Associations for Dental Research 2015.
PY - 2015/7/3
Y1 - 2015/7/3
N2 - Subgingival microorganisms are potentially associated with periodontal diseases. However, changes in the subgingival microbiota during the progress of periodontal diseases are poorly understood. In this study, we analyzed bacterial communities in the subgingival paper point samples from 32 Korean individuals with no sign of disease, gingivitis, or periodontitis using 454 FLX Titanium pyrosequencing. A total of 256,113 reads representing 26 phyla, 433 genera, and 1,016 species were detected. Bacteroidetes, Fusobacteria, Synergistetes, and Spirochaetes were the abundant phyla in periodontitis subjects, whereas Firmicutes and Proteobacteria were identified as the dominant phyla in the gingivitis and healthy subjects, respectively. Although high levels of Porphyromonas, Fusobacterium, Fretibacterium, Rothia, Filifactor, and Treponema genera were observed in the periodontitis subjects, Streptococcus, Capnocytophaga, Leptotrichia, and Haemophilus genera were found at high frequency in the gingivitis subjects. Species including Porphyromonas gingivalis, Fusobacterium nucleatum, and Fretibacterium fastidiosum were significantly increased in periodontitis subjects. On the other hand, Streptococcus pseudopneumoniae, Haemophilus parainfluenzae, and Leptotrichia hongkongensis were preferentially observed in the gingivitis subjects. Intriguingly, the halophile Halomonas hamiltonii was revealed as a predominant species in the healthy subjects. Based on Fast UniFrac analysis, distinctive bacterial clusters were classified for the healthy, gingivitis, and periodontitis state. The current findings might be useful for understanding the pathogenesis, diagnosis, and treatment of periodontal diseases.
AB - Subgingival microorganisms are potentially associated with periodontal diseases. However, changes in the subgingival microbiota during the progress of periodontal diseases are poorly understood. In this study, we analyzed bacterial communities in the subgingival paper point samples from 32 Korean individuals with no sign of disease, gingivitis, or periodontitis using 454 FLX Titanium pyrosequencing. A total of 256,113 reads representing 26 phyla, 433 genera, and 1,016 species were detected. Bacteroidetes, Fusobacteria, Synergistetes, and Spirochaetes were the abundant phyla in periodontitis subjects, whereas Firmicutes and Proteobacteria were identified as the dominant phyla in the gingivitis and healthy subjects, respectively. Although high levels of Porphyromonas, Fusobacterium, Fretibacterium, Rothia, Filifactor, and Treponema genera were observed in the periodontitis subjects, Streptococcus, Capnocytophaga, Leptotrichia, and Haemophilus genera were found at high frequency in the gingivitis subjects. Species including Porphyromonas gingivalis, Fusobacterium nucleatum, and Fretibacterium fastidiosum were significantly increased in periodontitis subjects. On the other hand, Streptococcus pseudopneumoniae, Haemophilus parainfluenzae, and Leptotrichia hongkongensis were preferentially observed in the gingivitis subjects. Intriguingly, the halophile Halomonas hamiltonii was revealed as a predominant species in the healthy subjects. Based on Fast UniFrac analysis, distinctive bacterial clusters were classified for the healthy, gingivitis, and periodontitis state. The current findings might be useful for understanding the pathogenesis, diagnosis, and treatment of periodontal diseases.
KW - bacteria
KW - dental plaque
KW - gingival crevicular fluid
KW - gingivitis
KW - periodontal pocket
KW - periodontitis
UR - http://www.scopus.com/inward/record.url?scp=84935145635&partnerID=8YFLogxK
U2 - 10.1177/0022034515583531
DO - 10.1177/0022034515583531
M3 - Article
C2 - 25904141
AN - SCOPUS:84935145635
SN - 0022-0345
VL - 94
SP - 921
EP - 927
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 7
ER -