Quantifying L-ascorbic acid-driven inhibitory effect on amyloid fibrillation

Wonseok Lee, Insu Kim, Sang Won Lee, Hyungbeen Lee, Gyudo Lee, Sangsig Kim, Sang Woo Lee, Dae Sung Yoon

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Ascorbic acid, which is widely used as a therapeutic agent for various disorders (e.g. chronic diseases and cancers), has potential therapeutic roles for neurodegenerative disease such as Huntington’s, Parkinson’s and Alzheimer’s diseases. The ability to impede amyloid fibril formation has a great demand on developing clinical medicine with respect to successfully preventing neurodegenerative diseases. Here, we report that L-ascorbic acid inhibits β-lactoglobulin amyloid formation in vitro. For quantitative characterization of the inhibitory effect of L-ascorbic acid on fibrillation, we performed high-resolution atomic force microscopy and thioflavin T fluorescence assay. Fourier transform infrared spectra indicated secondary structure differences of fibrils formed with and without ascorbic acid. These results suggest great potential of ascorbic acid for use in the prevention or treatment of amyloidogenic diseases.[Figure not available: see fulltext.]

    Original languageEnglish
    Pages (from-to)868-873
    Number of pages6
    JournalMacromolecular Research
    Volume24
    Issue number10
    DOIs
    Publication statusPublished - 2016 Oct 1

    Bibliographical note

    Publisher Copyright:
    © 2016, The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht.

    Keywords

    • L-ascorbic acid
    • atomic force microscopy
    • inhibition effect
    • thioflavin T fluorescence assay
    • β-lactoglobulin amyloid fibril

    ASJC Scopus subject areas

    • General Chemical Engineering
    • Organic Chemistry
    • Polymers and Plastics
    • Materials Chemistry

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