Quantifying L-ascorbic acid-driven inhibitory effect on amyloid fibrillation

Wonseok Lee, Insu Kim, Sang Won Lee, Hyungbeen Lee, Gyudo Lee, Sangsig Kim, Sang Woo Lee, Dae Sung Yoon

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Ascorbic acid, which is widely used as a therapeutic agent for various disorders (e.g. chronic diseases and cancers), has potential therapeutic roles for neurodegenerative disease such as Huntington’s, Parkinson’s and Alzheimer’s diseases. The ability to impede amyloid fibril formation has a great demand on developing clinical medicine with respect to successfully preventing neurodegenerative diseases. Here, we report that L-ascorbic acid inhibits β-lactoglobulin amyloid formation in vitro. For quantitative characterization of the inhibitory effect of L-ascorbic acid on fibrillation, we performed high-resolution atomic force microscopy and thioflavin T fluorescence assay. Fourier transform infrared spectra indicated secondary structure differences of fibrils formed with and without ascorbic acid. These results suggest great potential of ascorbic acid for use in the prevention or treatment of amyloidogenic diseases.[Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)868-873
Number of pages6
JournalMacromolecular Research
Issue number10
Publication statusPublished - 2016 Oct 1

Bibliographical note

Publisher Copyright:
© 2016, The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht.


  • L-ascorbic acid
  • atomic force microscopy
  • inhibition effect
  • thioflavin T fluorescence assay
  • β-lactoglobulin amyloid fibril

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


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