Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device

H. I. Choi; J. Y.H. Kim; J. W. Choi; S. J. Sim

Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device

H. I. Choi, J. Y.H. Kim, J. W. Choi, S. J. Sim

Department of Chemical and Biological Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Microalgae are capable of using bicarbonate (HCO₃^-) directly as an inorganic carbon species. Despite the importance of HCO₃^- in aquatic photosynthesis, little is known about the chemotaxis of microalgae towards HCO₃^-. Here, we demonstrated the chemotaxis of a model alga, Chlamydomonas reinhardtii, to HCO₃^- using an agarose gel-based zero-flow microfluidic device for generating a stable concentration gradient. We found C. reinhardtii showed the strongest chemotaxis towards HCO₃^- at the concentration of 26mM in the microfluidic system. We also observed that the various physiological conditions, such as circadian rhythm, mutation, and treatment of inhibitor have influence on the chemotaxis.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	1689-1690
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13

Publication series

Name	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	16/10/9 → 16/10/13

Bibliographical note

Funding Information:
This study was supported by Grant No. 2014M1A8A1049278 from Korea CCS R&D Center of the NRF funded by the Ministry of Science, ICT, and Future Planning of Korea, the National Research Foundation of Korea (NRF) (Grant No. NRF-2013R1A2A1A01015644/2010-0027955), the Korea Institute of Energy Technology Evaluation and Planning and Ministry of Trade, Industry and Energy of in "Energy Efficiency and Resources Technology R&D" project Korea (Grant No. 20152010201900), and University-Institute Cooperation Program (2013).

Keywords

Bicarbonate
Chemotaxis
Diffusion
Microalgae

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Choi, H. I., Kim, J. Y. H., Choi, J. W., & Sim, S. J. (2016). Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1689-1690). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device. / Choi, H. I.; Kim, J. Y.H.; Choi, J. W. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 1689-1690 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Choi, HI, Kim, JYH, Choi, JW & Sim, SJ 2016, Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 1689-1690, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.

Choi HI, Kim JYH, Choi JW, Sim SJ. Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 1689-1690. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Choi, H. I. ; Kim, J. Y.H. ; Choi, J. W. et al. / Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 1689-1690 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

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AU - Sim, S. J.

N1 - Funding Information: This study was supported by Grant No. 2014M1A8A1049278 from Korea CCS R&D Center of the NRF funded by the Ministry of Science, ICT, and Future Planning of Korea, the National Research Foundation of Korea (NRF) (Grant No. NRF-2013R1A2A1A01015644/2010-0027955), the Korea Institute of Energy Technology Evaluation and Planning and Ministry of Trade, Industry and Energy of in "Energy Efficiency and Resources Technology R&D" project Korea (Grant No. 20152010201900), and University-Institute Cooperation Program (2013).

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N2 - Microalgae are capable of using bicarbonate (HCO3-) directly as an inorganic carbon species. Despite the importance of HCO3- in aquatic photosynthesis, little is known about the chemotaxis of microalgae towards HCO3-. Here, we demonstrated the chemotaxis of a model alga, Chlamydomonas reinhardtii, to HCO3- using an agarose gel-based zero-flow microfluidic device for generating a stable concentration gradient. We found C. reinhardtii showed the strongest chemotaxis towards HCO3- at the concentration of 26mM in the microfluidic system. We also observed that the various physiological conditions, such as circadian rhythm, mutation, and treatment of inhibitor have influence on the chemotaxis.

AB - Microalgae are capable of using bicarbonate (HCO3-) directly as an inorganic carbon species. Despite the importance of HCO3- in aquatic photosynthesis, little is known about the chemotaxis of microalgae towards HCO3-. Here, we demonstrated the chemotaxis of a model alga, Chlamydomonas reinhardtii, to HCO3- using an agarose gel-based zero-flow microfluidic device for generating a stable concentration gradient. We found C. reinhardtii showed the strongest chemotaxis towards HCO3- at the concentration of 26mM in the microfluidic system. We also observed that the various physiological conditions, such as circadian rhythm, mutation, and treatment of inhibitor have influence on the chemotaxis.

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KW - Diffusion

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Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device

Abstract

Publication series

Other

Bibliographical note

Keywords

ASJC Scopus subject areas

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