Quantitative analysis of the interactions between 3d hepatocyte tissue and capillary networks in a microfluidic platform

Tomohiro Tsuji, Seok Chung, Roger Kamm, Mariko Ikeda, Kazuo Tanishita, Ryo Sudo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Tissue vascularization is one of the biggest challenges in tissue engineering. We constructed 3D hepatocyte tissue-like structures and endothelial capillary networks in a microfluidic platform. Here, we analyzed interactions between these structures quantitatively by recording the cell migration and morphogenesis. The analysis of capillary network extension rate under various combinations of growth factors (e.g., HGF, VEGF) revealed that the growth rate significantly increased by the growth factor addition. The capillary networks, however, stopped extending after contacting with hepatocytes, suggesting that additional factors are necessary for achieving hepatocyte tissue-like structures including microvascular networks.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1529-1531
Number of pages3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Hepatocyte
  • Tissue Engineering
  • Vascularization

ASJC Scopus subject areas

  • Control and Systems Engineering

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