TY - GEN
T1 - Quantitative analysis of the interactions between 3d hepatocyte tissue and capillary networks in a microfluidic platform
AU - Tsuji, Tomohiro
AU - Chung, Seok
AU - Kamm, Roger
AU - Ikeda, Mariko
AU - Tanishita, Kazuo
AU - Sudo, Ryo
PY - 2011
Y1 - 2011
N2 - Tissue vascularization is one of the biggest challenges in tissue engineering. We constructed 3D hepatocyte tissue-like structures and endothelial capillary networks in a microfluidic platform. Here, we analyzed interactions between these structures quantitatively by recording the cell migration and morphogenesis. The analysis of capillary network extension rate under various combinations of growth factors (e.g., HGF, VEGF) revealed that the growth rate significantly increased by the growth factor addition. The capillary networks, however, stopped extending after contacting with hepatocytes, suggesting that additional factors are necessary for achieving hepatocyte tissue-like structures including microvascular networks.
AB - Tissue vascularization is one of the biggest challenges in tissue engineering. We constructed 3D hepatocyte tissue-like structures and endothelial capillary networks in a microfluidic platform. Here, we analyzed interactions between these structures quantitatively by recording the cell migration and morphogenesis. The analysis of capillary network extension rate under various combinations of growth factors (e.g., HGF, VEGF) revealed that the growth rate significantly increased by the growth factor addition. The capillary networks, however, stopped extending after contacting with hepatocytes, suggesting that additional factors are necessary for achieving hepatocyte tissue-like structures including microvascular networks.
KW - Hepatocyte
KW - Tissue Engineering
KW - Vascularization
UR - http://www.scopus.com/inward/record.url?scp=84883823372&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883823372&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883823372
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1529
EP - 1531
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -