Abstract
A sandwich-type immunosensor composed of antigen-double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement of fluorescence intensity and quantitative analysis of single-stranded DNA based on the concentration of free glyphosate. The reaction between the antigen-double DNA-gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen-antibody reaction were measured on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 μg mL-1 for the detection of immobilized antigen-double DNA-gold NPs. For the quantitative analysis based on the concentration of glyphosate(0.01-100 μg mL-1), the immunosensor response also revealed the same detection range of glyphosate using DNA detection.
| Original language | English |
|---|---|
| Pages (from-to) | 12096-12100 |
| Number of pages | 5 |
| Journal | Journal of agricultural and food chemistry |
| Volume | 58 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - 2010 Dec 8 |
Keywords
- Immunosensor
- antigen-antibody
- fluorescence
- glyphosate
- gold nanoparticles
- quantitative analysis
ASJC Scopus subject areas
- General Chemistry
- General Agricultural and Biological Sciences