Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment

Jinseok Kim; Jungyul Park; Kyounghwan Na; Sungwook Yang; Jeongeun Baek; Euisung Yoon; Sungsik Choi; Sangho Lee; Kukjin Chun; Jongoh Park; Sukho Park

doi:10.1016/j.jbiomech.2008.05.036

Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment

Jinseok Kim, Jungyul Park, Kyounghwan Na, Sungwook Yang, Jeongeun Baek, Euisung Yoon, Sungsik Choi, Sangho Lee, Kukjin Chun, Jongoh Park, Sukho Park

* Honorary professors

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Three-dimensional cultures in a microfabricated environment provide in vivo-like conditions for cells, and have been used in a variety of applications in basic and clinical studies. In this study, the contractility of cardiomyocytes in a 3D environment using complex 3D hybrid biopolymer microcantilevers was quantified and compared with that observed in a 2D environment. By measuring the deflections of the microcantilevers with different surfaces and carrying out finite element modeling (FEM) of the focal pressures of the microcantilevers, it was found that the contractile force of high-density cardiomyocytes on 3D grooved surfaces was 65-85% higher than that of cardiomyocytes on flat surfaces. These results were supported by immunostaining, which showed alignment of the cytoskeleton and elongation of the nuclei, as well as by quantitative RT-PCR, which revealed that cells on the grooved surface had experienced sustained stimuli and tighter cell-to-cell interactions. Crown

Original language	English
Pages (from-to)	2396-2401
Number of pages	6
Journal	Journal of Biomechanics
Volume	41
Issue number	11
DOIs	https://doi.org/10.1016/j.jbiomech.2008.05.036
Publication status	Published - 2008 Aug

Keywords

3D microenvironment
Cardiomyocyte
Cell-to-cell interactions
Contractile force
Grooved surface
Microcantilever

ASJC Scopus subject areas

Biophysics
Biomedical Engineering
Orthopedics and Sports Medicine
Rehabilitation

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10.1016/j.jbiomech.2008.05.036

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title = "Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment",

abstract = "Three-dimensional cultures in a microfabricated environment provide in vivo-like conditions for cells, and have been used in a variety of applications in basic and clinical studies. In this study, the contractility of cardiomyocytes in a 3D environment using complex 3D hybrid biopolymer microcantilevers was quantified and compared with that observed in a 2D environment. By measuring the deflections of the microcantilevers with different surfaces and carrying out finite element modeling (FEM) of the focal pressures of the microcantilevers, it was found that the contractile force of high-density cardiomyocytes on 3D grooved surfaces was 65-85% higher than that of cardiomyocytes on flat surfaces. These results were supported by immunostaining, which showed alignment of the cytoskeleton and elongation of the nuclei, as well as by quantitative RT-PCR, which revealed that cells on the grooved surface had experienced sustained stimuli and tighter cell-to-cell interactions. Crown",

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note = "Funding Information: This research was supported by the Intelligent Microsystems Center, which funds many of the 21st Century's Frontier R&D Projects and the Next Generation New Technology Development Programs (No. 10030037) sponsored by the Korea Ministry of Commerce, Industry and Energy. ",

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AU - Yoon, Euisung

AU - Choi, Sungsik

AU - Lee, Sangho

AU - Chun, Kukjin

AU - Park, Jongoh

AU - Park, Sukho

N1 - Funding Information: This research was supported by the Intelligent Microsystems Center, which funds many of the 21st Century's Frontier R&D Projects and the Next Generation New Technology Development Programs (No. 10030037) sponsored by the Korea Ministry of Commerce, Industry and Energy.

PY - 2008/8

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N2 - Three-dimensional cultures in a microfabricated environment provide in vivo-like conditions for cells, and have been used in a variety of applications in basic and clinical studies. In this study, the contractility of cardiomyocytes in a 3D environment using complex 3D hybrid biopolymer microcantilevers was quantified and compared with that observed in a 2D environment. By measuring the deflections of the microcantilevers with different surfaces and carrying out finite element modeling (FEM) of the focal pressures of the microcantilevers, it was found that the contractile force of high-density cardiomyocytes on 3D grooved surfaces was 65-85% higher than that of cardiomyocytes on flat surfaces. These results were supported by immunostaining, which showed alignment of the cytoskeleton and elongation of the nuclei, as well as by quantitative RT-PCR, which revealed that cells on the grooved surface had experienced sustained stimuli and tighter cell-to-cell interactions. Crown

AB - Three-dimensional cultures in a microfabricated environment provide in vivo-like conditions for cells, and have been used in a variety of applications in basic and clinical studies. In this study, the contractility of cardiomyocytes in a 3D environment using complex 3D hybrid biopolymer microcantilevers was quantified and compared with that observed in a 2D environment. By measuring the deflections of the microcantilevers with different surfaces and carrying out finite element modeling (FEM) of the focal pressures of the microcantilevers, it was found that the contractile force of high-density cardiomyocytes on 3D grooved surfaces was 65-85% higher than that of cardiomyocytes on flat surfaces. These results were supported by immunostaining, which showed alignment of the cytoskeleton and elongation of the nuclei, as well as by quantitative RT-PCR, which revealed that cells on the grooved surface had experienced sustained stimuli and tighter cell-to-cell interactions. Crown

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Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment

Abstract

Keywords

ASJC Scopus subject areas

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