Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment

Jinseok Kim, Jungyul Park, Kyounghwan Na, Sungwook Yang, Jeongeun Baek, Euisung Yoon, Sungsik Choi, Sangho Lee, Kukjin Chun, Jongoh Park, Sukho Park

    Research output: Contribution to journalArticlepeer-review

    46 Citations (Scopus)

    Abstract

    Three-dimensional cultures in a microfabricated environment provide in vivo-like conditions for cells, and have been used in a variety of applications in basic and clinical studies. In this study, the contractility of cardiomyocytes in a 3D environment using complex 3D hybrid biopolymer microcantilevers was quantified and compared with that observed in a 2D environment. By measuring the deflections of the microcantilevers with different surfaces and carrying out finite element modeling (FEM) of the focal pressures of the microcantilevers, it was found that the contractile force of high-density cardiomyocytes on 3D grooved surfaces was 65-85% higher than that of cardiomyocytes on flat surfaces. These results were supported by immunostaining, which showed alignment of the cytoskeleton and elongation of the nuclei, as well as by quantitative RT-PCR, which revealed that cells on the grooved surface had experienced sustained stimuli and tighter cell-to-cell interactions. Crown

    Original languageEnglish
    Pages (from-to)2396-2401
    Number of pages6
    JournalJournal of Biomechanics
    Volume41
    Issue number11
    DOIs
    Publication statusPublished - 2008 Aug

    Bibliographical note

    Funding Information:
    This research was supported by the Intelligent Microsystems Center, which funds many of the 21st Century's Frontier R&D Projects and the Next Generation New Technology Development Programs (No. 10030037) sponsored by the Korea Ministry of Commerce, Industry and Energy.

    Keywords

    • 3D microenvironment
    • Cardiomyocyte
    • Cell-to-cell interactions
    • Contractile force
    • Grooved surface
    • Microcantilever

    ASJC Scopus subject areas

    • Biophysics
    • Biomedical Engineering
    • Orthopedics and Sports Medicine
    • Rehabilitation

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