TY - GEN
T1 - Quantitative live cell imaging with tomographic phase microscopy
AU - Choi, Wonshik
AU - Sung, Yongjin
AU - Park, Yongkeun
AU - Fang-Yen, Christopher
AU - Badizadegan, Kamran
AU - Dasari, Ramachandra R.
AU - Feld, Michael S.
PY - 2009
Y1 - 2009
N2 - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.
AB - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.
UR - http://www.scopus.com/inward/record.url?scp=85087192868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087192868&partnerID=8YFLogxK
U2 - 10.1364/ntm.2009.ntua1
DO - 10.1364/ntm.2009.ntua1
M3 - Conference contribution
AN - SCOPUS:85087192868
SN - 9781557528711
T3 - Optics InfoBase Conference Papers
BT - Novel Techniques in Microscopy, NTM 2009
PB - Optical Society of America (OSA)
T2 - Novel Techniques in Microscopy, NTM 2009
Y2 - 26 April 2009 through 30 April 2009
ER -
