Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi; Yongjin Sung; Yongkeun Park; Christopher Fang-Yen; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1364/ntm.2009.ntua1

Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi^*
, Yongjin Sung
, Yongkeun Park
, Christopher Fang-Yen
, Kamran Badizadegan
, Ramachandra R. Dasari
, Michael S. Feld

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

Original language	English
Title of host publication	Novel Techniques in Microscopy, NTM 2009
Publisher	Optical Society of America (OSA)
ISBN (Print)	9781557528711
DOIs	https://doi.org/10.1364/ntm.2009.ntua1
Publication status	Published - 2009
Event	Novel Techniques in Microscopy, NTM 2009 - Vancouver, Canada Duration: 2009 Apr 26 → 2009 Apr 30

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Other

Other	Novel Techniques in Microscopy, NTM 2009
Country/Territory	Canada
City	Vancouver
Period	09/4/26 → 09/4/30

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Instrumentation
Atomic and Molecular Physics, and Optics

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10.1364/ntm.2009.ntua1

Cite this

Choi, W, Sung, Y, Park, Y, Fang-Yen, C, Badizadegan, K, Dasari, RR & Feld, MS 2009, Quantitative live cell imaging with tomographic phase microscopy. in Novel Techniques in Microscopy, NTM 2009. Optics InfoBase Conference Papers, Optical Society of America (OSA), Novel Techniques in Microscopy, NTM 2009, Vancouver, Canada, 09/4/26. https://doi.org/10.1364/ntm.2009.ntua1

@inproceedings{bb9cb0c78d7b42e2bec3a2dae519ab8d,

title = "Quantitative live cell imaging with tomographic phase microscopy",

abstract = "In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.",

author = "Wonshik Choi and Yongjin Sung and Yongkeun Park and Christopher Fang-Yen and Kamran Badizadegan and Dasari, \{Ramachandra R.\} and Feld, \{Michael S.\}",

year = "2009",

doi = "10.1364/ntm.2009.ntua1",

language = "English",

isbn = "9781557528711",

series = "Optics InfoBase Conference Papers",

publisher = "Optical Society of America (OSA)",

booktitle = "Novel Techniques in Microscopy, NTM 2009",

note = "Novel Techniques in Microscopy, NTM 2009 ; Conference date: 26-04-2009 Through 30-04-2009",

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AU - Choi, Wonshik

AU - Sung, Yongjin

AU - Park, Yongkeun

AU - Fang-Yen, Christopher

AU - Badizadegan, Kamran

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

PY - 2009

Y1 - 2009

N2 - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

AB - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

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UR - https://www.scopus.com/pages/publications/85087192868#tab=citedBy

U2 - 10.1364/ntm.2009.ntua1

DO - 10.1364/ntm.2009.ntua1

M3 - Conference contribution

AN - SCOPUS:85087192868

SN - 9781557528711

T3 - Optics InfoBase Conference Papers

BT - Novel Techniques in Microscopy, NTM 2009

PB - Optical Society of America (OSA)

T2 - Novel Techniques in Microscopy, NTM 2009

Y2 - 26 April 2009 through 30 April 2009

ER -

Quantitative live cell imaging with tomographic phase microscopy

Abstract

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ASJC Scopus subject areas

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