Quantitative microfluidic assay to measure neutralizing and total antibodies for SARS-CoV-2

Hyunjun Bae, Dain Lee, Hana Kim, Young Sam Jee, Jian Kim, Byonggon Min, Ye Na Park, Joon Seok Seo, Jeong min Lee, Seongho Lee, Juhyung Kim, Han Sang Jo, Kyuhwan Na, Seok Chung, In Bum Suh

Research output: Contribution to journalArticlepeer-review

Abstract

Since the initial coronavirus disease (COVID-19) outbreak in 2019, humans have been adjusting to the pandemic era at the expense of adversity and death. This is due to the lack of definitive vaccines or effective treatments against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern. It is thus essential to assess the immune status of individuals to develop herd immunity. This study aimed to develop a rapid and quantitative antibody-detection-platform, targeting both neutralizing antibodies (NAbs) and total antibodies (TAbs) against SARS-CoV-2, by incorporating nano-interstice channels on a microfluidic chip with digitized flow control. The developed microfluidic platform demonstrated 100 % sensitivity and specificity for clinical samples (n = 38) from COVID-19-positive patients and healthy volunteers. This platform showed a strong correlation and agreement with the plaque-reduction neutralization test (PRNT), the gold standard for NAb assay. The novel assay demonstrated a powerful diagnostic performance, sufficient to replace PRNT or enzyme-linked immunosorbent assay (ELISA). The findings may contribute to COVID-19 management through continuous monitoring of the immune status of individuals and communities.

Original languageEnglish
Article number135093
JournalSensors and Actuators B: Chemical
Volume403
DOIs
Publication statusPublished - 2024 Mar 15

Bibliographical note

Publisher Copyright:
© 2023 Elsevier B.V.

Keywords

  • COVID-19
  • Microfluidics
  • Nano-interstice
  • Neutralizing antibodies
  • Point-of-Care Technology
  • SARS-CoV-2

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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