Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Seung Mok Ryu; Jaeyoung Kwon; Young Hye Seo; Eun Gyeong Song; Seong Su Hong; Beom Seok Kim; Jin Sung Hong; Ki Hyun Ryu; Dongho Lee

doi:10.1016/j.virusres.2016.09.013

Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Seung Mok Ryu, Jaeyoung Kwon, Young Hye Seo, Eun Gyeong Song, Seong Su Hong, Beom Seok Kim, Jin Sung Hong, Ki Hyun Ryu, Dongho Lee

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH₃OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10 μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40 μM.

Original language	English
Pages (from-to)	49-56
Number of pages	8
Journal	Virus Research
Volume	227
DOIs	https://doi.org/10.1016/j.virusres.2016.09.013
Publication status	Published - 2017 Jan 2

Keywords

Antiviral agent
Brucea javanica
Capsicum annuum
Green fluorescent protein
Pepper mottle virus
Quassinoid

ASJC Scopus subject areas

Virology
Infectious Diseases
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2016.09.013

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title = "Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper",

abstract = "A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10 μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40 μM.",

keywords = "Antiviral agent, Brucea javanica, Capsicum annuum, Green fluorescent protein, Pepper mottle virus, Quassinoid",

author = "Ryu, {Seung Mok} and Jaeyoung Kwon and Seo, {Young Hye} and Song, {Eun Gyeong} and Hong, {Seong Su} and Kim, {Beom Seok} and Hong, {Jin Sung} and Ryu, {Ki Hyun} and Dongho Lee",

note = "Funding Information: This work was supported by the iPET (Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries , 115090021SB010 ), the NRF (National Research Foundation of Korea , NRF-2015R1D1A1A01060321 and NRF-2015R1D1A1A01060614 ), and the Cooperative Research Program for Agriculture Science & Technology Development ( PJ01087804 ) in RDA. Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2017",

month = jan,

day = "2",

doi = "10.1016/j.virusres.2016.09.013",

language = "English",

volume = "227",

pages = "49--56",

journal = "Virus Research",

issn = "0168-1702",

publisher = "Elsevier",

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TY - JOUR

T1 - Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

AU - Ryu, Seung Mok

AU - Kwon, Jaeyoung

AU - Seo, Young Hye

AU - Song, Eun Gyeong

AU - Hong, Seong Su

AU - Kim, Beom Seok

AU - Hong, Jin Sung

AU - Ryu, Ki Hyun

AU - Lee, Dongho

N1 - Funding Information: This work was supported by the iPET (Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries , 115090021SB010 ), the NRF (National Research Foundation of Korea , NRF-2015R1D1A1A01060321 and NRF-2015R1D1A1A01060614 ), and the Cooperative Research Program for Agriculture Science & Technology Development ( PJ01087804 ) in RDA. Publisher Copyright: © 2016 Elsevier B.V.

PY - 2017/1/2

Y1 - 2017/1/2

N2 - A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10 μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40 μM.

AB - A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10 μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40 μM.

KW - Antiviral agent

KW - Brucea javanica

KW - Capsicum annuum

KW - Green fluorescent protein

KW - Pepper mottle virus

KW - Quassinoid

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DO - 10.1016/j.virusres.2016.09.013

M3 - Article

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AN - SCOPUS:84991830752

SN - 0168-1702

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SP - 49

EP - 56

JO - Virus Research

JF - Virus Research

ER -

Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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