Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Seung Mok Ryu, Jaeyoung Kwon, Young Hye Seo, Eun Gyeong Song, Seong Su Hong, Beom Seok Kim, Jin Sung Hong, Ki Hyun Ryu, Dongho Lee

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10 μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40 μM.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalVirus Research
Publication statusPublished - 2017 Jan 2

Bibliographical note

Funding Information:
This work was supported by the iPET (Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries , 115090021SB010 ), the NRF (National Research Foundation of Korea , NRF-2015R1D1A1A01060321 and NRF-2015R1D1A1A01060614 ), and the Cooperative Research Program for Agriculture Science & Technology Development ( PJ01087804 ) in RDA.

Publisher Copyright:
© 2016 Elsevier B.V.


  • Antiviral agent
  • Brucea javanica
  • Capsicum annuum
  • Green fluorescent protein
  • Pepper mottle virus
  • Quassinoid

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research


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