Radiation effect on 3D micro vascular network in in vitro microfluidic chip

Dong Hee Choi, Kyu Hwan Na, Hyunho Kim, Yong Hun Jung, Chun Ho Kim, Seok Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To demonstrate a radiation effect on vasculature, we designed 3D in vitro micro vascular system. We used soft-lithography method to fabricate in vitro microfluidic chip which was composed of two media channels and three gel channels. We cultured human umbilical vein endothelial cells(HUVECs) to make vasculature. To form a vasculature, fibrin gel was used as extracellular matrix to support HUVECs. After embedding, HUVECs were exposed to radiation on day 0 or 4. After irradiation, we analyzed structure of vasculature and measured gene expression. The results demonstrate a difference radioresistance between early stage of vasculature and mature stage of vasculature.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages60-61
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020 Jan 1
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period17/10/2217/10/26

Keywords

  • 3D vasculature
  • In vitro microfluidic chip
  • Radiation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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