Abstract
The aim of this study is the detection of atherosclerosis by using radiolabeled small peptides via non-invasive nuclear imaging. A small peptide, which shows high binding affinity for atherosclerotic lesions in vessels (AP), was synthesized by using a standard Fmoc method and diethylenetriaminepentaacetic acid (DTPA) or tyrosine was conjugated at the N-terminal of the AP to provide DTPA-AP-CC or Y-AP, respectively. The prepared peptide was labeled by " 99mTc, 111In or 123I. The labeled peptides were injected into atherosclerosis-induced mice (LDLr(-/-) knockout mice) and control mice via the tail vein. At 15 min post injection, the aortas of the LDLr(-/-) knockout and control mice were excised for an autoradiogram study. Gamma scintigraphic images were obtained at 30 min and 1 h. The optimized labeling yield of [ 99mTc]DTPA-AP-CC, [111In]DTPA-AP-CC and [ 123I]Y-AP was over 35%, 100% and over 20%, respectively. Autoradiographic studies showed that the aorta uptakes of [99mTc] DTPA-AP-CC and [111In]DTPA-AP-CC in the LDLr(-/-) knockout mice were much higher than the corresponding uptakes in the control mice. In contrast, the aorta uptake of [123I]Y-AP in LDLr(-/-) and normal mice were comparable. Scintigraphic images, however, did not show any noticeable uptake differences between the LDLr(-/-) knockout and the control mice, which is mostly due to the high background in the abdominal region and to the non-sufficient resolution of gamma camera imaging for the aorta of the mice.
Original language | English |
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Pages (from-to) | 858-864 |
Number of pages | 7 |
Journal | Journal of the Korean Physical Society |
Volume | 52 |
Issue number | 3 PART 1 |
DOIs | |
Publication status | Published - 2008 Mar |
Externally published | Yes |
Bibliographical note
Funding Information:This research was supported by the National Science Foundation under grant DMI 97-13718
Funding Information:
ACKNOWLEDGMENTS: This research was supported by the National Science Foundation under grant DMI 97-13718 and was conducted in the facilities of the Computer Integrated Manufacturing Laboratory, a constituent lab of the Institute for Systems Research. Jeffrey W. Herrmann has a joint appointment with the Institute for Systems Research. The authors appreciate the help provided by Dr. Peter Sandborn at the University of Maryland, College Park.
Keywords
- Atherosclerosis
- Autoradiogram
- I
- In
- Peptide
- Radiolabeling
- Tc
ASJC Scopus subject areas
- General Physics and Astronomy