RanBPM inhibits BLT2-mediated IL-8 production and invasiveness in aggressive breast cancer cells

Jun Dong Wei, Jae Hyun Jang, Jae Hong Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

RanBPM is a scaffolding protein that regulates several cellular processes by interacting with various proteins. Previously, we reported that RanBPM acts as a negative regulator of BLT2, a low-affinity leukotriene B4 receptor; thus, it interferes with BLT2-mediated cell motility. In the present study, we observed that the expression levels of RanBPM were markedly reduced in the highly aggressive MDA-MB-435 and MDA-MB-231 human breast cancer cell lines compared with those in non-invasive MCF-7 cells. Additionally, we found that the restoration of RanBPM levels suppressed the invasiveness of these aggressive breast cancer cells in a manner dependent on BLT2 activation. In contrast, the knockdown of endogenous RanBPM by shRNA strongly promoted invasiveness in non-invasive MCF-7 cells. We also observed that RanBPM suppressed the invasiveness of aggressive breast cancer cells by inhibiting BLT2-mediated reactive oxygen species (ROS) generation and IL-8 production. Taken together, our results suggest that RanBPM acts as a negative regulator of BLT2, thus attenuating the invasiveness of aggressive breast cancer cells.

Original languageEnglish
Pages (from-to)305-311
Number of pages7
JournalBiochemical and biophysical research communications
Volume483
Issue number1
DOIs
Publication statusPublished - 2017 Jan 29

Bibliographical note

Funding Information:
This work was supported by Bio & Medical Technology Development Program grants (2012M3A9C5048709 and 2012M3A9C1053532) and a Mid-Career Researcher Program grant (2015R1A2A1A15053311) through the National Research Foundation (NRF) funded by the Ministry of Science, Information and Communication Technologies (ICT) and Future Planning of the Republic of Korea. This work was also supported by Basic Science Research (2015R1D1A1A01057757) through the NRF funded by the Ministry of Education and by the BK21 Plus Program (College of Life Sciences and Biotechnology, Korea University), as well as a Korea University Grant.

Publisher Copyright:
© 2016 Elsevier Inc.

Keywords

  • BLT2
  • Breast cancer
  • IL-8
  • Invasiveness
  • RanBPM

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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