Rapid analysis of barley straw before and after dilute sulfuric acid pretreatment by photoluminescence

Sung Bong Kim, Chunzhi Cui, Ja Hyun Lee, Sang Jun Lee, Dong June Ahn, Chulhwan Park, Jun Seok Kim, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The fluorescence intensities (FIs) of raw and pretreated barley straws were measured by fluorescence microscopy, and the difference in the fluorescence intensity of barley straw before and after dilute acid pretreatment was analyzed by investigation of the major compounds of barley straw. The difference in fluorescence intensity was due to the difference in xylan content. Barley straw was pretreated using dilute sulfuric acid at various conditions and the correlation between the fluorescence intensity and glucose yield of barley straw was investigated. The coefficient of determination (R2) of the correlation was found to be 72.28%. Also the calibration of fluorescence intensity with the xylan content was performed. In addition, the absorption and emission spectra of the raw and the pretreated barley straw were examined to verify the proposed method. The absorption and emission wave lengths were 550nm and 665nm, respectively.

Original languageEnglish
Pages (from-to)789-793
Number of pages5
JournalBioresource technology
Publication statusPublished - 2013 Oct

Bibliographical note

Funding Information:
This study was supported by Technology Development Program ( 309016-5 ) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and the Advanced Biomass R&D Center ( ABC-2010-0029799 ) of Korea Grant funded by the Ministry of Education, Science and Technology .


  • Biomass analysis
  • Dilute acid pretreatment
  • Fluorescence
  • Photoluminescence
  • Xylan

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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