Rapid and efficient isolation of exosomes by clustering and scattering

Jinhyun Kim, Hoyoon Lee, Kyonghwa Park, Sehyun Shin

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Tumor-derived extracellular vesicles (EVs) have become important biomarkers of liquid biopsies for precision medicine. However, the clinical application of EVs has been limited due to the lack of EV isolation practical technology applicable to clinical environments. Here, we report an innovative EV isolation method, which is quick and simple, and facilitates high-yield and high-purity EV isolation from blood. Introducing a cationic polymer in plasma resulted in rapid clustering of anionic EVs and a chaotropic agent can separate EVs from these clusters. Isolated EVs were characterized in terms of size distribution, morphology, surface protein markers, and exosomal RNA. Through performance comparison with various methods, including ultracentrifugation (UC), the present method delivered the highest recovery rate (~20 folds that of UC) and purity ratio (3.5 folds that of UC) of EVs in a short period of time (<20 min). The proposed method is expected to be used in basic and applied research on EV isolation and in clinical applications.

Original languageEnglish
Article number650
JournalJournal of Clinical Medicine
Issue number3
Publication statusPublished - 2020 Mar

Bibliographical note

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.


  • Blood
  • Isolation
  • Liquid biopsy
  • Polymer
  • Tumor-derive extracellular vesicles

ASJC Scopus subject areas

  • General Medicine


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