TY - JOUR
T1 - Rapid colorimetric analysis of multiple microRNAs using encoded hydrogel microparticles
AU - Kim, Ju Yeon
AU - Mun, Seok Joon
AU - Roh, Yoon Ho
AU - Bong, Ki Wan
N1 - Funding Information:
This research was supported by the National Research Foundation of Korea (NRF), funded by the Korean Government Ministry of Science and ICT (MSIT, Korea) (grant number 2016R1A5A1010148) and the Next-Generation Biogreen 21 Program funded by the Rural Development Administration of the Republic of Korea (no. PJ016004). This research was also supported by the Basic Science Research Program through the NRF, funded by the Ministry of Education (NRF-2018R1D1A1B07046577).
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2021/9/21
Y1 - 2021/9/21
N2 - microRNAs (miRNAs) have attracted much attention as potential biomarkers for the diagnosis of various fatal diseases. With increasing interest in miRNA detection at practical sites, colorimetric bead-based assays have garnered much attention, because these allow for simple analysis with cheap and portable devices. Among them, the encoded hydrogel microparticle-based colorimetric miRNA assay is considered as one of the promising techniques, due to its strengths, such as large multiplex capacity, acceptable sensitivity, and simple analysis. However, it still imposes a limitation in terms of the assay time, particularly the colorimetric reaction time, which is too long, making the practical application of the assay difficult and undermining its detection accuracy. In this work, we present a rapid colorimetric assay based on encoded hydrogel microparticles, which exhibits a significant decrease in the colorimetric reaction time due to two factors: (1) an increase in the number of enzymes bound to hydrogel microparticles via a post-synthesis functionalization method, and (2) an elevation in the enzyme reaction temperature during colorimetric labeling. We obtained a comparable sensitivity of the colorimetric assay with three different miRNA targets, even with a shortened colorimetric reaction time. Furthermore, we validated that our colorimetric detection method is suitable for multiplex miRNA detection, owing to its low cross-reactivity. This journal is
AB - microRNAs (miRNAs) have attracted much attention as potential biomarkers for the diagnosis of various fatal diseases. With increasing interest in miRNA detection at practical sites, colorimetric bead-based assays have garnered much attention, because these allow for simple analysis with cheap and portable devices. Among them, the encoded hydrogel microparticle-based colorimetric miRNA assay is considered as one of the promising techniques, due to its strengths, such as large multiplex capacity, acceptable sensitivity, and simple analysis. However, it still imposes a limitation in terms of the assay time, particularly the colorimetric reaction time, which is too long, making the practical application of the assay difficult and undermining its detection accuracy. In this work, we present a rapid colorimetric assay based on encoded hydrogel microparticles, which exhibits a significant decrease in the colorimetric reaction time due to two factors: (1) an increase in the number of enzymes bound to hydrogel microparticles via a post-synthesis functionalization method, and (2) an elevation in the enzyme reaction temperature during colorimetric labeling. We obtained a comparable sensitivity of the colorimetric assay with three different miRNA targets, even with a shortened colorimetric reaction time. Furthermore, we validated that our colorimetric detection method is suitable for multiplex miRNA detection, owing to its low cross-reactivity. This journal is
UR - http://www.scopus.com/inward/record.url?scp=85115130600&partnerID=8YFLogxK
U2 - 10.1039/d1an00622c
DO - 10.1039/d1an00622c
M3 - Article
C2 - 34346406
AN - SCOPUS:85115130600
SN - 0003-2654
VL - 146
SP - 5508
EP - 5516
JO - The Analyst
JF - The Analyst
IS - 18
ER -