Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene

Kyoung Sik Han; Younghoon Kim; Seungkook Choi; Sejong Oh; Sungsu Park; Sae Hun Kim; Kwang Youn Whang

doi:10.1007/s10529-005-0014-z

Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene

Kyoung Sik Han, Younghoon Kim, Seungkook Choi, Sejong Oh, Sungsu Park, Sae Hun Kim, Kwang Youn Whang

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.

Original language	English
Pages (from-to)	1183-1188
Number of pages	6
Journal	Biotechnology letters
Volume	27
Issue number	16
DOIs	https://doi.org/10.1007/s10529-005-0014-z
Publication status	Published - 2005 Aug

Keywords

Intergenic spacer region
Lactobacillus acidophilus
Restriction fragment length polymorphism

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-005-0014-z

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title = "Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene",

abstract = "A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.",

keywords = "Intergenic spacer region, Lactobacillus acidophilus, Restriction fragment length polymorphism",

author = "Han, {Kyoung Sik} and Younghoon Kim and Seungkook Choi and Sejong Oh and Sungsu Park and Kim, {Sae Hun} and Whang, {Kwang Youn}",

note = "Funding Information: This study was supported by a Grant of Korea University, Republic of Korea (K0179500).",

year = "2005",

month = aug,

doi = "10.1007/s10529-005-0014-z",

language = "English",

volume = "27",

pages = "1183--1188",

journal = "Biotechnology Letters",

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TY - JOUR

T1 - Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene

AU - Han, Kyoung Sik

AU - Kim, Younghoon

AU - Choi, Seungkook

AU - Oh, Sejong

AU - Park, Sungsu

AU - Kim, Sae Hun

AU - Whang, Kwang Youn

N1 - Funding Information: This study was supported by a Grant of Korea University, Republic of Korea (K0179500).

PY - 2005/8

Y1 - 2005/8

N2 - A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.

AB - A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.

KW - Intergenic spacer region

KW - Lactobacillus acidophilus

KW - Restriction fragment length polymorphism

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SN - 0141-5492

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SP - 1183

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 16

ER -

Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene

Abstract

Keywords

ASJC Scopus subject areas

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