Rapid induction of edible lipids in Chlorella by mild electric stimulation

S. A. Choi; Soo Youn Lee; Jiye Lee; Jun Muk Cho; Jin Suk Lee; Seung Wook Kim; Dong Yeon Kim; Se Kook Park; Chang Soo Jin; You Kwan Oh

doi:10.1016/j.biortech.2019.121950

Rapid induction of edible lipids in Chlorella by mild electric stimulation

S. A. Choi, Soo Youn Lee, Jiye Lee, Jun Muk Cho, Jin Suk Lee, Seung Wook Kim, Dong Yeon Kim, Se Kook Park, Chang Soo Jin, You Kwan Oh

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

In this work, a new stress-based method for rapid induction of triacylglycerol (TAG) and total and polyunsaturated fatty acid accumulations in Chlorella sp. by mild electric stimulation is presented. When a cathodic current of 31 mA (voltage: 4 V) was applied to the algal cells for 4 h, the TAG content of the electro-treated cells was sharply increased to a level 2.1 times that of the untreated control. The contents of the polyunsaturated linoleic (C18:2n6) and linolenic (C18:3n3) acids in the electro-treated cells were also 36 and 57% higher than those in the untreated cells, respectively. Cyclic voltammetry and various biochemical analyses indicate that TAG and fatty acid formations are electro-stimulated via de novo fatty acid biosynthesis and metabolic transformation in the Chlorella cells.

Original language	English
Article number	121950
Journal	Bioresource technology
Volume	292
DOIs	https://doi.org/10.1016/j.biortech.2019.121950
Publication status	Published - 2019 Nov

Bibliographical note

Publisher Copyright:
© 2019

Keywords

Chlorella
Electric stimulation
Essential polyunsaturated fatty acids
Lipid induction
Triacylglycerol

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.121950

Cite this

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title = "Rapid induction of edible lipids in Chlorella by mild electric stimulation",

abstract = "In this work, a new stress-based method for rapid induction of triacylglycerol (TAG) and total and polyunsaturated fatty acid accumulations in Chlorella sp. by mild electric stimulation is presented. When a cathodic current of 31 mA (voltage: 4 V) was applied to the algal cells for 4 h, the TAG content of the electro-treated cells was sharply increased to a level 2.1 times that of the untreated control. The contents of the polyunsaturated linoleic (C18:2n6) and linolenic (C18:3n3) acids in the electro-treated cells were also 36 and 57% higher than those in the untreated cells, respectively. Cyclic voltammetry and various biochemical analyses indicate that TAG and fatty acid formations are electro-stimulated via de novo fatty acid biosynthesis and metabolic transformation in the Chlorella cells.",

keywords = "Chlorella, Electric stimulation, Essential polyunsaturated fatty acids, Lipid induction, Triacylglycerol",

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year = "2019",

month = nov,

doi = "10.1016/j.biortech.2019.121950",

language = "English",

volume = "292",

journal = "Bioresource technology",

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AU - Choi, S. A.

AU - Lee, Soo Youn

AU - Lee, Jiye

AU - Cho, Jun Muk

AU - Lee, Jin Suk

AU - Kim, Seung Wook

AU - Kim, Dong Yeon

AU - Park, Se Kook

AU - Jin, Chang Soo

AU - Oh, You Kwan

PY - 2019/11

Y1 - 2019/11

N2 - In this work, a new stress-based method for rapid induction of triacylglycerol (TAG) and total and polyunsaturated fatty acid accumulations in Chlorella sp. by mild electric stimulation is presented. When a cathodic current of 31 mA (voltage: 4 V) was applied to the algal cells for 4 h, the TAG content of the electro-treated cells was sharply increased to a level 2.1 times that of the untreated control. The contents of the polyunsaturated linoleic (C18:2n6) and linolenic (C18:3n3) acids in the electro-treated cells were also 36 and 57% higher than those in the untreated cells, respectively. Cyclic voltammetry and various biochemical analyses indicate that TAG and fatty acid formations are electro-stimulated via de novo fatty acid biosynthesis and metabolic transformation in the Chlorella cells.

AB - In this work, a new stress-based method for rapid induction of triacylglycerol (TAG) and total and polyunsaturated fatty acid accumulations in Chlorella sp. by mild electric stimulation is presented. When a cathodic current of 31 mA (voltage: 4 V) was applied to the algal cells for 4 h, the TAG content of the electro-treated cells was sharply increased to a level 2.1 times that of the untreated control. The contents of the polyunsaturated linoleic (C18:2n6) and linolenic (C18:3n3) acids in the electro-treated cells were also 36 and 57% higher than those in the untreated cells, respectively. Cyclic voltammetry and various biochemical analyses indicate that TAG and fatty acid formations are electro-stimulated via de novo fatty acid biosynthesis and metabolic transformation in the Chlorella cells.

KW - Chlorella

KW - Electric stimulation

KW - Essential polyunsaturated fatty acids

KW - Lipid induction

KW - Triacylglycerol

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DO - 10.1016/j.biortech.2019.121950

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AN - SCOPUS:85071997806

SN - 0960-8524

VL - 292

JO - Bioresource technology

JF - Bioresource technology

M1 - 121950

ER -

Rapid induction of edible lipids in Chlorella by mild electric stimulation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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