Abstract
A rapid method for the determination of 14 types of isoflavones in food using ultra-high performance liquid chromatography (UHPLC) was validated in terms of precision, accuracy, sensitivity and linearity. The UHPLC separation was performed on a reverse-phase C18 column (particle size 2 μm, i.d. 2 mm, length 100 mm) using a photo diode array detector that was fixed to 260 nm. The limits of detection and quantification of the UHPLC analyses ranged from 0.03 to 0.33 mg kg-1. The intra-day and inter-day precision of the individual isoflavones were less than 11.77% and calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. These results suggest that the rapid method used in this study could be available to determine of 14 types of isoflavones in a variety of food such as soy bean, black bean, red bean and soybean paste.
Original language | English |
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Pages (from-to) | 391-397 |
Number of pages | 7 |
Journal | Food Chemistry |
Volume | 187 |
DOIs | |
Publication status | Published - 2015 Nov 15 |
Bibliographical note
Publisher Copyright:© 2015 Elsevier Ltd.
Keywords
- Isoflavone
- Method validation
- Rapid method
- UHPLC
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science