Rapid quantification of cellular flavonoid levels using quercetin and a fluorescent diphenylboric acid 2-amino ethyl ester probe

Ji Hae Lee; Yeojin Kim; Minh Hien Hoang; Hee jin Jun; Sung Joon Lee

doi:10.1007/s10068-014-0010-y

Rapid quantification of cellular flavonoid levels using quercetin and a fluorescent diphenylboric acid 2-amino ethyl ester probe

Ji Hae Lee, Yeojin Kim, Minh Hien Hoang, Hee jin Jun, Sung Joon Lee

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Flavonoids are abundant in fruits and vegetables and have various biological activities. A rapid, sensitive method for assessing cellular quercetin levels is necessary because cellular levels are often low. A new method for measuring cellular flavonoid levels is proposed using diphenylboric acid 2-aminoethyl ester (DPBA), a fluorescent probe, and quercetin, a representative flavonoid. This spectrofluorometry-based assay is simple and allows rapid quantification of the cellular quercetin content with high sensitivity. CHO-K1 cells were incubated with quercetin for 5 min, and then the cellular quercetin concentration was assayed using DPBA spectrofluorometry. Results, compared with high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and conventional spectrophotometry measurements, were highly correlated with HPLC data (R>0.99). The method is more sensitive than TLC and conventional spectrophotometry. Spectrofluorometric measurement using DPBA is a simple, rapid, and sensitive method for assessing cellular quercetin levels.

Original language	English
Pages (from-to)	75-79
Number of pages	5
Journal	Food Science and Biotechnology
Volume	23
Issue number	1
DOIs	https://doi.org/10.1007/s10068-014-0010-y
Publication status	Published - 2014 Feb

Bibliographical note

Funding Information:
Acknowledgments This study was supported by the “Global Ph.D. Fellowship project” of the National Research Foundation of Korea (NRF), and by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ008506)” of the Rural Development Administration, Republic of Korea.

Keywords

diphenylboric acid 2-aminoethyl ether (DPBA)
flavonoids
fluorescence activated cell sorter (FACS)
quercetin
spectrofluorometry

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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title = "Rapid quantification of cellular flavonoid levels using quercetin and a fluorescent diphenylboric acid 2-amino ethyl ester probe",

abstract = "Flavonoids are abundant in fruits and vegetables and have various biological activities. A rapid, sensitive method for assessing cellular quercetin levels is necessary because cellular levels are often low. A new method for measuring cellular flavonoid levels is proposed using diphenylboric acid 2-aminoethyl ester (DPBA), a fluorescent probe, and quercetin, a representative flavonoid. This spectrofluorometry-based assay is simple and allows rapid quantification of the cellular quercetin content with high sensitivity. CHO-K1 cells were incubated with quercetin for 5 min, and then the cellular quercetin concentration was assayed using DPBA spectrofluorometry. Results, compared with high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and conventional spectrophotometry measurements, were highly correlated with HPLC data (R>0.99). The method is more sensitive than TLC and conventional spectrophotometry. Spectrofluorometric measurement using DPBA is a simple, rapid, and sensitive method for assessing cellular quercetin levels.",

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author = "Lee, {Ji Hae} and Yeojin Kim and Hoang, {Minh Hien} and Jun, {Hee jin} and Lee, {Sung Joon}",

note = "Funding Information: Acknowledgments This study was supported by the “Global Ph.D. Fellowship project” of the National Research Foundation of Korea (NRF), and by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ008506)” of the Rural Development Administration, Republic of Korea.",

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AU - Kim, Yeojin

AU - Hoang, Minh Hien

AU - Jun, Hee jin

AU - Lee, Sung Joon

N1 - Funding Information: Acknowledgments This study was supported by the “Global Ph.D. Fellowship project” of the National Research Foundation of Korea (NRF), and by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ008506)” of the Rural Development Administration, Republic of Korea.

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N2 - Flavonoids are abundant in fruits and vegetables and have various biological activities. A rapid, sensitive method for assessing cellular quercetin levels is necessary because cellular levels are often low. A new method for measuring cellular flavonoid levels is proposed using diphenylboric acid 2-aminoethyl ester (DPBA), a fluorescent probe, and quercetin, a representative flavonoid. This spectrofluorometry-based assay is simple and allows rapid quantification of the cellular quercetin content with high sensitivity. CHO-K1 cells were incubated with quercetin for 5 min, and then the cellular quercetin concentration was assayed using DPBA spectrofluorometry. Results, compared with high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and conventional spectrophotometry measurements, were highly correlated with HPLC data (R>0.99). The method is more sensitive than TLC and conventional spectrophotometry. Spectrofluorometric measurement using DPBA is a simple, rapid, and sensitive method for assessing cellular quercetin levels.

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Rapid quantification of cellular flavonoid levels using quercetin and a fluorescent diphenylboric acid 2-amino ethyl ester probe

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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