Rapid quantification of lipids in acremonium chrysogenum using Oil red O

Hyun Yong Shin; Jin Young Lee; Eun Ji Kim; Seung Wook Kim

doi:10.1007/s00284-010-9818-8

Rapid quantification of lipids in acremonium chrysogenum using Oil red O

Hyun Yong Shin, Jin Young Lee, Eun Ji Kim, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml^-1 of lipids in A. chrysogenum.

Original language	English
Pages (from-to)	1023-1027
Number of pages	5
Journal	Current Microbiology
Volume	62
Issue number	3
DOIs	https://doi.org/10.1007/s00284-010-9818-8
Publication status	Published - 2011 Mar

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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abstract = "A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml-1 of lipids in A. chrysogenum.",

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AU - Lee, Jin Young

AU - Kim, Eun Ji

AU - Kim, Seung Wook

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AB - A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml-1 of lipids in A. chrysogenum.

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Rapid quantification of lipids in acremonium chrysogenum using Oil red O

Abstract

ASJC Scopus subject areas

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