Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction

Min Eui Hong, Hong Il Choi, Ho Seok Kwak, Sung Won Hwang, Young Joon Sung, Won Seok Chang, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalBioresource technology
Volume267
DOIs
Publication statusPublished - 2018 Nov

Bibliographical note

Funding Information:
The authors are grateful to Hoang-Minh Pham for thoughtful technical advice. The authors also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278) and National Research Foundation of Korea (NRF) funded by the Korea government (grant number: NRF-2016R1A2A1A05005465) and Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government (Ministry of Trade, Industry and Energy) (grant number: 20172010202050). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC-1003542018S075) and Korea University.

Funding Information:
The authors are grateful to Hoang-Minh Pham for thoughtful technical advice. The authors also would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278) and National Research Foundation of Korea ( NRF ) funded by the Korea government (grant number: NRF-2016R1A2A1A05005465 ) and Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government ( Ministry of Trade, Industry and Energy ) (grant number: 20172010202050 ). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation ( KDHC ) (grant number: KDHC-1003542018S075 ) and Korea University .

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

  • Autotrophic astaxanthin production
  • Azide-based colorimetric assay
  • Haematococcus pluvialis
  • High-throughput screening
  • Oil-based astaxanthin extraction method

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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