RASSF1A suppresses the activated K-Ras-induced oxidative DNA damage

Seon Ho Park; Jung Jin Kim; Jin Sil Chung; So Ra Lee; Gi Young Lee; Hyung Jung Kim; Young Do Yoo

doi:10.1016/j.bbrc.2011.03.139

RASSF1A suppresses the activated K-Ras-induced oxidative DNA damage

Seon Ho Park, Jung Jin Kim, Jin Sil Chung, So Ra Lee, Gi Young Lee, Hyung Jung Kim, Young Do Yoo

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The mutant K-Ras elevates intracellular reactive oxygen species (ROS) levels and leads to oxidative DNA damage, resulting in malignant cell transformation. Ras association domain family 1 isoform A (RASSF1A) is known to play a role as a Ras effector. However, the suppressive effect of RASSF1A on K-RasV12-induced ROS increase and DNA damage has not been identified. Here, we show that RASSF1A blocks K-RasV12-triggered ROS production. RASSF1A expression also inhibits oxidative DNA damage and chromosomal damage. From the results obtained in this study, we suggest that RASSF1A regulates the cellular ROS levels enhanced by the Ras signaling pathway, and that it may function as a tumor suppressor by suppressing DNA damage caused by activated Ras.

Original language	English
Pages (from-to)	149-153
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	408
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2011.03.139
Publication status	Published - 2011 Apr 29

Keywords

DNA damage
K-Ras
RASSF1A
ROS

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2011.03.139

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title = "RASSF1A suppresses the activated K-Ras-induced oxidative DNA damage",

abstract = "The mutant K-Ras elevates intracellular reactive oxygen species (ROS) levels and leads to oxidative DNA damage, resulting in malignant cell transformation. Ras association domain family 1 isoform A (RASSF1A) is known to play a role as a Ras effector. However, the suppressive effect of RASSF1A on K-RasV12-induced ROS increase and DNA damage has not been identified. Here, we show that RASSF1A blocks K-RasV12-triggered ROS production. RASSF1A expression also inhibits oxidative DNA damage and chromosomal damage. From the results obtained in this study, we suggest that RASSF1A regulates the cellular ROS levels enhanced by the Ras signaling pathway, and that it may function as a tumor suppressor by suppressing DNA damage caused by activated Ras.",

keywords = "DNA damage, K-Ras, RASSF1A, ROS",

author = "Park, {Seon Ho} and Kim, {Jung Jin} and Chung, {Jin Sil} and Lee, {So Ra} and Lee, {Gi Young} and Kim, {Hyung Jung} and Yoo, {Young Do}",

note = "Funding Information: This research was supported by a grant of The Korea Healthcare technology R&D Project , Ministry for Health, Welfare & Family Affairs , Republic of Korea ( A084537-0902-0000100 ).",

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AU - Park, Seon Ho

AU - Kim, Jung Jin

AU - Chung, Jin Sil

AU - Lee, So Ra

AU - Lee, Gi Young

AU - Kim, Hyung Jung

AU - Yoo, Young Do

N1 - Funding Information: This research was supported by a grant of The Korea Healthcare technology R&D Project , Ministry for Health, Welfare & Family Affairs , Republic of Korea ( A084537-0902-0000100 ).

PY - 2011/4/29

Y1 - 2011/4/29

N2 - The mutant K-Ras elevates intracellular reactive oxygen species (ROS) levels and leads to oxidative DNA damage, resulting in malignant cell transformation. Ras association domain family 1 isoform A (RASSF1A) is known to play a role as a Ras effector. However, the suppressive effect of RASSF1A on K-RasV12-induced ROS increase and DNA damage has not been identified. Here, we show that RASSF1A blocks K-RasV12-triggered ROS production. RASSF1A expression also inhibits oxidative DNA damage and chromosomal damage. From the results obtained in this study, we suggest that RASSF1A regulates the cellular ROS levels enhanced by the Ras signaling pathway, and that it may function as a tumor suppressor by suppressing DNA damage caused by activated Ras.

AB - The mutant K-Ras elevates intracellular reactive oxygen species (ROS) levels and leads to oxidative DNA damage, resulting in malignant cell transformation. Ras association domain family 1 isoform A (RASSF1A) is known to play a role as a Ras effector. However, the suppressive effect of RASSF1A on K-RasV12-induced ROS increase and DNA damage has not been identified. Here, we show that RASSF1A blocks K-RasV12-triggered ROS production. RASSF1A expression also inhibits oxidative DNA damage and chromosomal damage. From the results obtained in this study, we suggest that RASSF1A regulates the cellular ROS levels enhanced by the Ras signaling pathway, and that it may function as a tumor suppressor by suppressing DNA damage caused by activated Ras.

KW - DNA damage

KW - K-Ras

KW - RASSF1A

KW - ROS

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RASSF1A suppresses the activated K-Ras-induced oxidative DNA damage

Abstract

Keywords

ASJC Scopus subject areas

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