Ratiometric detection of mitochondrial thiols with a two-photon fluorescent probe

Chang Su Lim; Goutam Masanta; Hyung Joong Kim; Ji Hee Han; Hwan Myung Kim; Bong Rae Cho

doi:10.1021/ja205081s

Ratiometric detection of mitochondrial thiols with a two-photon fluorescent probe

Chang Su Lim, Goutam Masanta, Hyung Joong Kim, Ji Hee Han, Hwan Myung Kim, Bong Rae Cho

* Honorary professors

Research output: Contribution to journal › Article › peer-review

341 Citations (Scopus)

Abstract

We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90 - 190 |m depth without interference from other biologically relevant species through the use of two-photon microscopy.

Original language	English
Pages (from-to)	11132-11135
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	133
Issue number	29
DOIs	https://doi.org/10.1021/ja205081s
Publication status	Published - 2011 Jul 27

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja205081s

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abstract = "We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90 - 190 |m depth without interference from other biologically relevant species through the use of two-photon microscopy.",

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AU - Masanta, Goutam

AU - Kim, Hyung Joong

AU - Han, Ji Hee

AU - Kim, Hwan Myung

AU - Cho, Bong Rae

PY - 2011/7/27

Y1 - 2011/7/27

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AB - We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90 - 190 |m depth without interference from other biologically relevant species through the use of two-photon microscopy.

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Ratiometric detection of mitochondrial thiols with a two-photon fluorescent probe

Abstract

ASJC Scopus subject areas

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