We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90 - 190 |m depth without interference from other biologically relevant species through the use of two-photon microscopy.
|Number of pages||4|
|Journal||Journal of the American Chemical Society|
|Publication status||Published - 2011 Jul 27|
ASJC Scopus subject areas
- Colloid and Surface Chemistry