Rck1 up-regulates pseudohyphal growth by activating the Ras2 and MAP kinase pathways independently in Saccharomyces cerevisiae

Miwha Chang, Chang Min Kang, Yong Sung Park, Cheol Won Yun

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Previously, we reported that Rck1 regulates Hog1 and Slt2 activities and affects MAP kinase activity in Saccharomyces cerevisiae. Recently, we found that Rck1 up-regulates phospho-Kss1 and phospho-Fus3. Kss1 has been known as a component in the pseudohyphal growth pathway, and we attempted to identify the function of Rck1 in pseudohyphal growth. Rck1 up-regulated Ras2 at the protein level, not the transcriptional level. Additionally, FLO11 transcription was up-regulated by RCK1 over-expression. RCK1 expression was up-regulated during growth on SLAD + 1% butanol medium. On nitrogen starvation agar plates, RCK1 over-expression induced pseudohyphal growth of colonies, and cells over-expressing RCK1 showed a filamentous morphology when grown in SLAD medium. Furthermore, 1-butanol greatly induced filamentous growth when RCK1 was over-expressed. Moreover, invasive growth was activated in haploid cells when RCK1 was over-expressed. The growth defect of cells observed on 1-butanol medium was recovered when RCK1 was over-expressed. Interestingly, Ras2 and phospho-Kss1 were up-regulated by Rck1 independently. Together, these results suggest that Rck1 promotes pseudohyphal growth by activating Ras2 and Kss1 via independent pathways in S. Cerevisiae.

    Original languageEnglish
    Pages (from-to)656-661
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume444
    Issue number4
    DOIs
    Publication statusPublished - 2014 Feb 21

    Bibliographical note

    Funding Information:
    This work was supported by a grant from the National Research Foundation of Korea (NRF) and funded by the Korean government (MEST) (No. 2012-0007043 ).

    Keywords

    • Kss1
    • MAP kinase
    • Ras2
    • Rck1
    • S. Cerevisiae

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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