Re-Analysis of 16S Amplicon Sequencing Data Reveals Soil Microbial Population Shifts in Rice Fields under Drought Condition

Seok Won Jang, Myeong Hyun Yoou, Woo Jong Hong, Yeon Ju Kim, Eun Jin Lee, Ki Hong Jung

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Rice (Oryza sativa. L) has been intensively studied to ensure a stable global supply of this commodity in the face of rapid global climate change. A critical factor that decreases crop yield is drought, which has been analyzed in various ways through many researches. Microbiome-based studies of rice investigate the symbiosis between rice and bacteria, which has been proposed as a way to overcome problems caused by drought. Several rice-associated metagenomic profiles obtained under drought conditions have been reported since the advent of next generation sequencing (NGS) technology. To elucidate the future diversity of plants and microorganisms and to promote sustainable agriculture, we reanalyzed 64 of the publicly available 16S amplicon sequencing data produced under drought condition. In the process of integrating data sets, however, we found an inconsistency that serves as a bottleneck for microbiome-based sustainability research. While this report provides clues about the composition of the microbiome under the drought conditions, the results are affected by differences in the location of the experiments, sampling conditions, and analysis protocols. Re-analysis of amplicon sequencing data of the soil microbiome in rice fields suggests that microbial composition shifts in response to drought condition and the presence of plants. Among the bacteria involved, the phylum Proteobacteria appears to play the most important role in the survival of rice under drought condition.

Original languageEnglish
Article number44
Issue number1
Publication statusPublished - 2020 Dec 1

Bibliographical note

Funding Information:
This work was supported by grants from the Next Generation BioGreen 21 Program (PJ01325901 and PJ01366401 to KHJ); National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (2018R1A4A1025158 to KHJ, and 2016R1A2B2012424 to EJL), and Global Ph.D. Fellowship Program supported by the NRF (NRF-2018H1A2A1060336 to WJH). Acknowledgments

Publisher Copyright:
© 2020, The Author(s).


  • Amplicon sequencing
  • Drought
  • Meta-analysis
  • Microbiome
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science


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