Re-engineering of genetic circuit for 2-deoxystreptamine (2-DOS) biosynthesis in Escherichia coli BL21 (DE3)

Amit Kumar Chaudhary, Je Won Park, Yeo Joon Yoon, Byung Gee Kim, Jae Kyung Sohng

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Various approaches for monocistronic constructions of genetic circuits have been designed for metabolite production but there has been no attempt to apply such methodology for aminoglycosides biosynthesis. Here, a simple and commercially available bio-part, despite the current trend focusing on the standardized BioBricks bio-parts available in the registry, is used. A 181-bp nucleotide fragment was designed for the efficient construction of an expression vector for monocistronic assembly of genes. Furthermore, a single vector with multi-monocistronic assembled genes for 2-deoxystreptamine (2-DOS) synthesis was constructed for production in engineered Escherichia coli. The working efficiency of model vector was concluded by reporter assay whereas the expressions of biosynthesis genes were confirmed by RT-PCR and SDS-PAGE. Production of 2-DOS was confirmed by TLC, LC-ELSD, and ESI-MS/MS.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalBiotechnology letters
Issue number2
Publication statusPublished - 2013 Feb
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments This study was supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031960) and by the grant from the Next-Generation BioGreen 21 Program (PJ008013), Rural Development Administration, and Republic of Korea.


  • Multi-monocistronic
  • Polycistronic
  • Pseudo-operon
  • Re-engineering of genetic circuit
  • Synthetic biology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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