Real-time imaging of glioblastoma using bioluminescence in a U-87 MG xenograft model mouse

Woong Kim; Bo Ram Kang; Hye Yun Kim; Soo Min Cho; Yong Deok Lee; Sehoon Kim; Jung Young Kim; Dong Jin Kim; Young Soo Kim

doi:10.1007/s13765-015-0037-7

Real-time imaging of glioblastoma using bioluminescence in a U-87 MG xenograft model mouse

Woong Kim, Bo Ram Kang, Hye Yun Kim, Soo Min Cho, Yong Deok Lee, Sehoon Kim, Jung Young Kim, Dong Jin Kim, Young Soo Kim

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Glioblastoma multiforme (GBM), the most common malignant brain tumor, is characterized by aggressive proliferation and invasive potential. Xenograft animal models of GBM have critically contributed to evaluation of novel therapeutic agents, drug delivery system, and diagnostic tools. To mimic intrinsic behavior of GBM, orthotopic transplantation of cancer cells and continuous observation of cell growth should be conducted in animal study. Here, we generated xenograft model mouse of GBM in which U-87 MG human glioblastoma cells were intracranially implanted for live imaging. Introducing luciferase gene into U-87 MG cell line enabled real-time observation and quantification of tumor survival and propagation by detecting photon emission derived from luciferase. Our GBM model mouse has potentials to bring great advantages in pharmacological and mechanistic investigation on brain tumors.

Original language	English
Pages (from-to)	243-248
Number of pages	6
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume	58
Issue number	2
DOIs	https://doi.org/10.1007/s13765-015-0037-7
Publication status	Published - 2015 Apr

Bibliographical note

Publisher Copyright:
© 2015, The Korean Society for Applied Biological Chemistry.

Keywords

Bioluminescence imaging
Glioblastoma
U-87 MG
Xenograft

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s13765-015-0037-7

Cite this

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title = "Real-time imaging of glioblastoma using bioluminescence in a U-87 MG xenograft model mouse",

abstract = "Glioblastoma multiforme (GBM), the most common malignant brain tumor, is characterized by aggressive proliferation and invasive potential. Xenograft animal models of GBM have critically contributed to evaluation of novel therapeutic agents, drug delivery system, and diagnostic tools. To mimic intrinsic behavior of GBM, orthotopic transplantation of cancer cells and continuous observation of cell growth should be conducted in animal study. Here, we generated xenograft model mouse of GBM in which U-87 MG human glioblastoma cells were intracranially implanted for live imaging. Introducing luciferase gene into U-87 MG cell line enabled real-time observation and quantification of tumor survival and propagation by detecting photon emission derived from luciferase. Our GBM model mouse has potentials to bring great advantages in pharmacological and mechanistic investigation on brain tumors.",

keywords = "Bioluminescence imaging, Glioblastoma, U-87 MG, Xenograft",

author = "Woong Kim and Kang, {Bo Ram} and Kim, {Hye Yun} and Cho, {Soo Min} and Lee, {Yong Deok} and Sehoon Kim and Kim, {Jung Young} and Kim, {Dong Jin} and Kim, {Young Soo}",

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doi = "10.1007/s13765-015-0037-7",

language = "English",

volume = "58",

pages = "243--248",

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T1 - Real-time imaging of glioblastoma using bioluminescence in a U-87 MG xenograft model mouse

AU - Kim, Woong

AU - Kang, Bo Ram

AU - Kim, Hye Yun

AU - Cho, Soo Min

AU - Lee, Yong Deok

AU - Kim, Sehoon

AU - Kim, Jung Young

AU - Kim, Dong Jin

AU - Kim, Young Soo

PY - 2015/4

Y1 - 2015/4

N2 - Glioblastoma multiforme (GBM), the most common malignant brain tumor, is characterized by aggressive proliferation and invasive potential. Xenograft animal models of GBM have critically contributed to evaluation of novel therapeutic agents, drug delivery system, and diagnostic tools. To mimic intrinsic behavior of GBM, orthotopic transplantation of cancer cells and continuous observation of cell growth should be conducted in animal study. Here, we generated xenograft model mouse of GBM in which U-87 MG human glioblastoma cells were intracranially implanted for live imaging. Introducing luciferase gene into U-87 MG cell line enabled real-time observation and quantification of tumor survival and propagation by detecting photon emission derived from luciferase. Our GBM model mouse has potentials to bring great advantages in pharmacological and mechanistic investigation on brain tumors.

AB - Glioblastoma multiforme (GBM), the most common malignant brain tumor, is characterized by aggressive proliferation and invasive potential. Xenograft animal models of GBM have critically contributed to evaluation of novel therapeutic agents, drug delivery system, and diagnostic tools. To mimic intrinsic behavior of GBM, orthotopic transplantation of cancer cells and continuous observation of cell growth should be conducted in animal study. Here, we generated xenograft model mouse of GBM in which U-87 MG human glioblastoma cells were intracranially implanted for live imaging. Introducing luciferase gene into U-87 MG cell line enabled real-time observation and quantification of tumor survival and propagation by detecting photon emission derived from luciferase. Our GBM model mouse has potentials to bring great advantages in pharmacological and mechanistic investigation on brain tumors.

KW - Bioluminescence imaging

KW - Glioblastoma

KW - U-87 MG

KW - Xenograft

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U2 - 10.1007/s13765-015-0037-7

DO - 10.1007/s13765-015-0037-7

M3 - Article

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SN - 1738-2203

VL - 58

SP - 243

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JO - Journal of the Korean Society for Applied Biological Chemistry

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ER -

Real-time imaging of glioblastoma using bioluminescence in a U-87 MG xenograft model mouse

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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