Abstract
Proteases are involved in almost all biological processes, and therefore, aberrant activity of many of these enzymes is an important indicator of disease. Various methods have been developed to analyze protease activity, among which, protease assays based on resonance energy transfer are currently used most widely. However, quantitative methods with relatively higher sensitivity are needed, especially for disease diagnosis at early stages. One of the strategies to achieve higher sensitivity is to implement signal amplification of the protease activity. In this review, we briefly summarize the protease assay methods based on resonance energy transfer, and then elaborate the efforts to develop sensitive protease assays through signal amplification by using enzyme cascades.
| Original language | English |
|---|---|
| Pages (from-to) | 1113-1119 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 17 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2012 |
| Externally published | Yes |
Bibliographical note
Funding Information:The authors acknowledge financial support from the New Faculty Research Program funded by Ajou University, the Basic Science Research Program (2012001787), and the Priority Research Centers Program (2012-0006687) through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology.
Keywords
- Enzyme cascade
- Protease assay
- Resonance energy transfer
- Signal amplification
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering